高原地区成年藏绵羊和小尾寒羊附睾组织中eNOS的差异表达研究

发布时间：2018-02-24 05:22

本文关键词： eNOS 藏绵羊小尾寒羊附睾免疫组织化学SP法　出处：《中国兽医科学》2017年05期 　论文类型：期刊论文

【摘要】：采用免疫组化SP法结合IPP统计分析法,比较高原地区成年藏绵羊和小尾寒羊附睾组织中eNOS的定位及分布特征。结果显示,eNOS在藏绵羊及小尾寒羊附睾各部分间质及血管壁均有表达,藏绵羊管腔上皮未见表达,而在小尾寒羊主要定位于附睾各部分管腔上皮。平均光密度值统计表明,eNOS在藏绵羊附睾尾部显著高于附睾头和附睾体(P0.05),小尾寒羊附睾体eNOS的表达显著高于藏绵羊(P0.05)。本研究提示在高原低氧环境中eNOS参与血管舒缩调节附睾局部微循环对氧的摄取;eNOS在小尾寒羊附睾管腔上皮的强表达可能是附睾微环境低氧应激反应的一个方面,它在高原地区动物附睾的调节机制有待于进一步研究。
[Abstract]:Immunohistochemical SP method combined with IPP statistical analysis was used. The localization and distribution of eNOS in the epididymis of Tibetan sheep and small tail Han sheep were compared at high altitude. The results showed that Enos was expressed in the interstitial and vascular wall of epididymis of Tibetan sheep and small tail Han sheep, but not in lumen epithelium of Tibetan sheep. But in small tail Han sheep, it was mainly located in the lumen epithelium of epididymis. The average optical density showed that the expression of Enos in the tail of epididymis of Tibetan sheep was significantly higher than that of head of epididymis and P0.05 of epididymis, and the expression of eNOS in epididymis of small tail Han sheep was significantly higher than that of Tibetan sheep. This study suggests that the strong expression of eNOS in the epididymal epithelium of small tail Han sheep may be one of the aspects of hypoxic stress in the epididymal microenvironment, which is involved in the regulation of oxygen uptake by vasomotor and contraction in the local microcirculation of epididymis. The regulatory mechanism of the epididymis in plateau animals needs to be further studied.
【作者单位】：甘肃农业大学动物医学院;
【基金】：国家自然科学基金项目(31160488) 甘肃农业大学青年导师基金项目(GAU-QDNS-201604) 甘肃省财政厅基本科研业务项目(2012)
【分类号】：S826

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