一种新型仔猪先天性震颤及其病原的初步研究

发布时间：2018-02-24 14:21

本文关键词： 仔猪先天性震颤瘟病毒仔猪先天性震颤瘟病毒(APPV) 序列分析　出处：《中国畜牧兽医》2017年07期 　论文类型：期刊论文

【摘要】：为了解国内猪群是否存在新型仔猪先天性震颤及其病原,本试验对临床疑似仔猪先天性震颤病例进行剖检,观察组织脏器的病理变化;根据GenBank上登录的瘟病毒基因组序列设计2对引物,以典型震颤仔猪的病料RNA为模板,进行RT-PCR,并对阳性产物进行测序;用DNAStar和Mega 7.0软件对所测序列进行分析,绘制系统进化树。结果表明,疑似仔猪先天性震颤病猪的组织脏器病理变化与猪瘟的病理变化相似,但无明显的脾脏梗死和淋巴结周边出血;从采集的病料中均扩增到与预期大小基本一致的条带;所测序列经BLAST比对,均为仔猪先天性震颤瘟病毒(APPV)的相应基因序列片段;系统进化树结果显示,哺乳动物瘟病毒可分为两大进化分支,APPV为单独一分支,其他瘟病毒为另一分支,两分支的同源性低于70%;所测的NS2-3和NS5B基因序列与GenBank上登录的APPV相应序列同源性在76.9%~98.8%之间,表明本研究所测定的序列是APPV的基因组序列;测定的7条NS2-3基因序列之间的同源性在89.7%~94.8%之间,12条NS5B基因序列的同源性在82.2%~100.0%之间。本试验首次证实国内猪群中存在新型仔猪先天性震颤病及其病原(APPV)。
[Abstract]:In order to find out whether there is a new type of congenital tremor and its pathogen in domestic piglets, the clinical suspected cases of congenital tremor in piglets were dissected and the pathological changes of tissues and organs were observed. Two pairs of primers were designed according to the genome sequence of plague virus registered on GenBank. RT-PCRs were used as template of RNA, and the positive products were sequenced. The sequences were analyzed by DNAStar and Mega 7.0 software. The phylogenetic tree was plotted. The results showed that the pathological changes of tissues and organs of pigs suspected of congenital tremor were similar to those of classical swine fever, but there was no obvious splenic infarction and peripheral hemorrhage of lymph nodes. All the samples were amplified with the expected size, the sequences were sequenced by BLAST alignment, and the phylogenetic tree showed that the sequence was the corresponding gene fragment of Piglet congenital tremor plague virus (APPVV), and the phylogenetic tree showed that the sequence was similar to the expected size, and the results of phylogenetic tree showed that, Mammal plague virus can be divided into two major evolutionary branches: APPV is a single branch, and the other is another branch. The homology of the two branches is less than 70. The homology of the detected NS2-3 and NS5B genes with the corresponding sequence of APPV registered on GenBank is 76.9% or 98.8%. The results showed that the sequence determined in this study was the genomic sequence of APPV. The homology of the 7 NS2-3 gene sequences was between 89.78% and 94.8%. The homology of 12 NS5B genes was between 82.2% and 100.0%.
【作者单位】：江西农业大学动物科学技术学院;江西金伊博生物科技有限公司;
【基金】：江西省科技支撑计划项目(20121BBF60031) 江西省现代农业产业技术体系建设专项资金(JXARS-03)
【分类号】：S858.28

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