MUC4和MUC13基因在ETECF18抗性型和易感型梅山断奶仔猪间的差异表达分析

发布时间：2018-02-26 07:42

  本文关键词： 猪 MUC基因 MUC基因 产肠毒素大肠杆菌F　出处：《中国畜牧兽医》2017年04期 　论文类型：期刊论文

【摘要】：MUC4和MUC13基因作为产肠毒素大肠杆菌(enterotoxigenic Escherichia coil,ETEC)F4的重要抗性候选基因,可能在断奶仔猪抗ETEC F18菌株的感染过程中发挥重要作用,因此本试验运用实时荧光定量PCR法分析了MUC4和MUC13基因在梅山猪ETEC F18抗性型和易感型个体11个组织(心脏、肝脏、脾脏、肺脏、肾脏、胃、肌肉、胸腺、淋巴结、十二指肠和空肠)中的表达水平及差异。结果显示,MUC4和MUC13基因在检测的11个组织中均有不同程度的表达。其中,在胸腺和淋巴组织中,MUC4基因在抗性型个体中的表达水平显著高于易感型个体(P0.05);在肺脏组织中,MUC13基因在抗性型个体中的表达水平显著高于易感型个体(P0.05),且MUC13基因在抗性群体肠道中的表达水平较高。由此推测,MUC4基因在免疫组织、MUC13基因在肠道组织的高表达可在一定程度上提高了机体的免疫能力,保护和润滑肠道,从而抵抗ETEC F18菌株对机体的感染。
[Abstract]:MUC4 and MUC13 genes, which are important candidate genes for enterotoxigenic Escherichia Coilogenic Escherichia ETECF4, may play an important role in the process of weaning piglets resistant to ETEC F18. Therefore, real-time fluorescence quantitative PCR was used to analyze 11 tissues (heart, liver, spleen, lung, kidney, stomach, muscle, thymus, lymph node) of ETEC F18 resistant and susceptible individuals of Meishan pig. The expression levels and differences in duodenum and jejunum. The results showed that MUC4 and MUC13 genes were expressed to different degrees in 11 tissues. The expression level of MUC4 gene in thymocytes and lymphoid tissues was significantly higher than that in susceptible individuals (P 0.05), and in lung tissues was significantly higher than that in susceptible individuals (P 0.05), and the expression of MUC13 gene in susceptible individuals was significantly higher than that in susceptible individuals. The high expression of MUC4 gene in the immune tissue could improve the immune ability of the body to some extent, so the high expression of MUC13 gene in the intestinal tissue of the resistant population was higher than that of the control group, which suggested that the high expression of MUC4 gene in the immune tissue could improve the immune ability of the body. Protect and lubricate the intestines against ETEC F 18 infection.
【作者单位】： 扬州大学江苏省动物遗传繁育与分子设计重点实验室;江苏省太仓市畜牧兽医站;
【基金】：江苏省科技支撑计划重点项目(BE2014357) 苏州市科技局攻关项目(SNG201628)
【分类号】：S828
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本文编号：1537151