柠檬苦素对牛前体脂肪细胞分化过程中核纤层蛋白基因表达的影响

发布时间：2018-02-27 17:11

本文关键词： 牛核纤层蛋白柠檬苦素脂肪沉积基因表达　出处：《黑龙江畜牧兽医》2017年03期 　论文类型：期刊论文

【摘要】：核纤层位于核膜内侧,主要由核纤层蛋白构成,对细胞核起重要的机械支持作用。编码核纤层蛋白的基因突变能导致人类肌肉、脂肪等组织病变,统称为核纤层病。为了研究柠檬苦素对牛前体脂肪细胞分化过程中核纤层蛋白基因表达的影响,试验利用qRT-PCR技术分析了编码核纤层蛋白的LMNA、LMNB1和LMNB2基因在牛前体脂肪细胞诱导分化过程中的表达变化,同时在细胞培养基中添加柠檬苦素,以分析柠檬苦素对细胞分化和核纤层蛋白编码基因表达的影响。结果表明:在培养基中添加柠檬苦素能够有效抑制牛前体脂肪细胞向成熟脂肪细胞的分化,试验组细胞中脂质沉积量极显著低于对照组(P0.01)。LMNA基因在牛前体脂肪细胞中具有较高的表达水平,添加柠檬苦素的试验组LMNA基因表达水平有降低的趋势。试验组LMNB1和LMNB2基因表达量在诱导分化的第0.5天极显著低于对照组(P0.01),在诱导分化的10~12 d试验组LMNB1基因表达量显著高于对照组(P0.05)。LMNA、LMNB1和LMNB2基因在牛前体脂肪细胞诱导分化过程中具有相似的表达变化趋势,均在诱导分化开始的0.5~1 d表达量升高。说明柠檬苦素在牛前体脂肪细胞早期分化时具有抑制核纤层蛋白基因表达的作用,而在分化后期则能够促进核纤层蛋白基因表达。
[Abstract]:The nuclear fiber layer is located on the inner side of the nuclear membrane and is mainly composed of nuclear laminin, which plays an important role in mechanically supporting the nucleus. The mutation of the gene encoding the nuclear laminin can lead to human muscle, fat and other tissue lesions. In order to study the effect of limonin on the expression of nuclear fiber laminin gene during the differentiation of bovine precursor adipocytes, QRT-PCR technique was used to analyze the expression changes of LMNAN LMNB1 and LMNB2 genes in bovine precursor adipocytes, and limonin was added to cell culture medium. In order to analyze the effect of limonin on cell differentiation and the expression of nuclear laminin coding gene, the results showed that the addition of limonin in culture medium could effectively inhibit the differentiation of bovine precursor adipocytes into mature adipocytes. The level of lipid deposition in the experimental group was significantly lower than that in the control group, and the expression level of LMNA gene in bovine precursor adipocytes was higher than that in the control group. The expression of LMNB1 and LMNB2 genes in the experimental group was significantly lower than that in the control group on the 0.5 day after differentiation, and the expression of LMNB1 gene in the experimental group was significantly lower than that in the control group on the 10th and 12th day after inducing differentiation. The expression of LMNB1 and LMNB2 genes in the preadipocytes was significantly higher than that in the control group. There was a similar trend in the expression of LMNB1 and LMNB2 in the differentiation of bovine preadipocytes. These results indicated that limonin could inhibit the expression of nuclear laminin gene in the early differentiation of bovine precursor adipocytes, while it could promote the expression of nuclear laminin gene at the late stage of differentiation.
【作者单位】：中国热带农业科学院热带作物品种资源研究所;河南科技大学动物科技学院;
【基金】：国家自然科学基金项目(31301941)
【分类号】：S823

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