液相色谱-串联质谱法同时测定动物血浆中21种霉菌毒素或其代谢物残留

发布时间：2018-02-27 18:37

本文关键词： 液相色谱-串联质谱动物血浆霉菌毒素残留　出处：《分析化学》2017年02期 　论文类型：期刊论文

【摘要】：建立了同时检测动物血浆中黄曲霉毒素B1等21种霉菌毒素或其代谢物残留的液相色谱-串联质谱方法。动物血浆样品中加入0.1%甲酸-乙腈溶液、NaCl和无水MgSO_4进行萃取,无水MgSO_4和C_(18),PSA,A-AL对提取液进行脱水净化,经浓缩、复溶和离心后,再进行测定。采用反相C_(18)色谱柱分离,以0.1%甲酸-0.5 mmol/L乙酸铵溶液和0.1%甲酸-甲醇溶液作为流动相进行梯度洗脱,采用电喷雾离子源(ESI)多反应监测离子模式(MRM)进行检测,基质标准曲线外标法进行定量分析,线性范围在0.05~100 ng/mL之间,方法的定量限为0.05~0.5 ng/mL。在高、中、低3个添加浓度水平下,21种霉菌毒素的平均回收率为62.0%~116.4%,相对标准偏差小于19%。
[Abstract]:A liquid chromatography-tandem mass spectrometry (LC-MS) method for simultaneous determination of aflatoxin B1 and its metabolites in animal plasma was established. 0.1% formic acrylonitrile solution and anhydrous MgSO_4 were added to the plasma samples for extraction. Anhydrous MgSO_4 and CSAAAL were used for dehydration and purification of the extract, then concentrated, redissolved and centrifuged, and then determined. The column was separated by reversed phase C _ (18) column, and the gradient elution was carried out with 0.1% formic acid -0.5 mmol/L ammonium acetate solution and 0.1% formate-methyl alcohol solution as mobile phase. The electrospray ion source (ESI) multi-reaction monitoring ion mode was used for the detection, and the matrix standard curve external standard method was used for quantitative analysis. The linear range was between 0.05 and 100 ng/mL, and the quantitative limit of the method was 0.05 ~ 0.5ng / mL. The average recovery of 21 mycotoxins was 62.0%, 116.4%, and the relative standard deviation was less than 19.4%.
【作者单位】：中国农业科学院农业质量标准与检测技术研究所农业部农产品质量安全研究重点实验室;奥特奇生物制品(中国)有限公司;
【基金】："十二五"农村领域国家科技计划(No.2011BAD26B0405) 公益性行业(农业)科研专项经费(No.201203088-01)资助~~
【分类号】：S859.8;O657.63

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