二甲双胍对犬乳腺肿瘤细胞周期及其相关蛋白的影响

发布时间：2018-02-28 05:03

本文关键词： 二甲双胍犬乳腺肿瘤细胞周期 cyclin D p　出处：《畜牧与兽医》2017年04期 　论文类型：期刊论文

【摘要】：为研究二甲双胍对犬乳腺肿瘤细胞增殖和细胞周期的影响,以犬乳腺肿瘤CHMm和CHMp为模型,采用终浓度为0、5、10、20、40mmol/L二甲双胍对其进行干预,应用CCK-8法检测细胞增殖活性,流式细胞仪检测细胞周期分布比例,再通过Western blot检测细胞周期相关蛋白cyclin D1和p27的表达情况。二甲双胍作用48 h后,与对照组比较,CHMm细胞试验各组的成活率显著降低(P0.05),而CHMp细胞除了5 mmol/L组(P0.05),其他试验各组的成活率也显著低于对照组(P0.05),并且均呈现一定的浓度依赖性。流式细胞仪分析细胞分布比例发现,CHMm的G0/G1期细胞比例从(38.7±5.89)%增加到(70.36±5.78)%,CHMp的G0/G1期细胞比例也从(37.03±4.23)%增加到(54.92±4.67)%。随着二甲双胍作用浓度的增加,CHMm和CHMp细胞内cyclin D1表达量呈现减少的趋势,而p27表达量呈现上升的趋势。研究结果表明,二甲双胍可以抑制犬乳腺肿瘤细胞体外增殖能力,诱导细胞发生G0/G1期阻滞,并下调cyclin D1和上调p27细胞周期相关蛋白表达量,从而为犬乳腺肿瘤治疗提供一定的理论基础。
[Abstract]:In order to study the effect of metformin on the proliferation and cell cycle of canine mammary tumor cells, CHMm and CHMp were used as models, and the final concentration of 0 5 ~ (10) 10 ~ (20) 渭 mol / L metformin was used to investigate the effect of metformin on the proliferation of canine breast tumor cells. The cell proliferation activity was detected by CCK-8 assay. Flow cytometry was used to detect the cell cycle distribution and the expression of cell cycle related proteins cyclin D1 and p27 was detected by Western blot. Metformin was treated with metformin for 48 h. Compared with the control group, the survival rate of CHMp cells in each group was significantly lower than that in the control group. The survival rate of CHMp cells was significantly lower than that of the control group in a concentration dependent manner, except for the 5 mmol/L group. Flow cytometry showed that the survival rate of the other groups was significantly lower than that of the control group. The percentage of G _ 0 / G _ 1 phase cells increased from 38.7 卤5.89% to 70.36 卤5.78% from 37.03 卤4.23% to 54.92 卤4.67%. The expression of cyclin D1 decreased with the increase of metformin concentration. The results showed that metformin could inhibit the proliferation of canine breast tumor cells, induce G _ 0 / G _ 1 arrest, down-regulate cyclin D1 and up-regulate the expression of p27 cell cycle related proteins. It provides a theoretical basis for the treatment of canine breast tumor.
【作者单位】：东北农业大学动物医学学院;
【基金】：国家自然基金(31372492和31672617) 国家重点研究项目(2016YFD0501008)
【分类号】：S858.292

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