MC1R基因在不同毛色太行山羊皮肤组织中的表达

发布时间：2018-02-28 05:31

本文关键词： 太行山羊 MCR基因序列特征 mRNA表达　出处：《中国畜牧杂志》2017年03期 　论文类型：期刊论文

【摘要】：本研究旨在研究太行山羊MC1R基因在不同毛色皮肤组织中的表达规律。运用RT-PCR方法从纯黑色太行山羊皮肤组织中扩增了MC1R基因的编码区序列,运用生物信息学方法分析了其编码蛋白的结构特点,利用实时荧光定量PCR技术,对4种不同毛色(纯黑、纯白、黄褐、黑斑白)各4只太行山羊,共计16个个体的皮肤组织进行mRNA表达研究。结果表明:MC1R基因CDS区长954 bp,编码317个氨基酸,其编码蛋白是G蛋白偶联受体家族的成员;同源性比对和进化树结果显示,与绵羊、家牛和水牛同源性较高;荧光定量结果显示,MC1 RmRNA在纯黑色个体皮肤组织中表达量最高,黄褐色次之。本研究结果为进一步研究该基因在毛色形成中的作用以及对纯黑色太行山羊的纯繁选育奠定基础。
[Abstract]:The purpose of this study was to study the expression of MC1R gene in different skin tissues of Taihang Shan sheep and amplify the coding region of MC1R gene from pure black Taihang mountain sheep skin tissue by RT-PCR method. The structural characteristics of the encoded protein were analyzed by bioinformatics method. Four different fur colors (pure black, pure white, yellow brown, black spot white) and four Taihang mountain sheep were studied by real-time fluorescence quantitative PCR. The expression of mRNA in skin tissues of 16 individuals was studied. The results showed that the CDS region of the 1% MC1R gene was 954 BP long, encoding 317 amino acids, and the encoding protein was a member of the G protein-coupled receptor family. The results of fluorescence quantitative analysis showed that the expression of MC1 RmRNA was the highest in the skin of pure black individuals. The results laid a foundation for the further study of the role of the gene in the formation of coat color and the breeding of pure black Taihang mountain sheep.
【作者单位】：山西农业大学动物科技学院;山西省原平农业学校;
【基金】：山西省科技攻关项目(20120312010-2) 科技部863子课题(2011AA100307-05) 山西省研究生优秀创新项目(2015SY27)
【分类号】：S827

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