口服黄芪多糖增强肠道黏膜免疫及其对口蹄疫疫苗免疫的影响

发布时间：2018-02-28 10:30

本文关键词： 黄芪多糖黏膜免疫口蹄疫疫苗　出处：《中国兽医学报》2017年03期 　论文类型：期刊论文

【摘要】：将40只小鼠随机分为2组,一组小鼠灌服黄芪多糖水溶液,另一组灌服生理盐水为对照组,给药后每周从各组随机取5只小鼠,分离脾淋巴细胞进行淋巴细胞增殖试验,并观察十二指肠黏膜上皮内淋巴细胞(IELs)以及上皮固有层的IgA+细胞数;将24只小鼠随机分为4组,各小鼠分2次,间隔2周注射口蹄疫(FMD)疫苗,其中1~3组的小鼠在疫苗免疫前灌服不同剂量的黄芪多糖,第4组小鼠灌服生理盐水作为对照。二免后1~5周,每周采血,检测血清IgG及其亚类。结果表明,小鼠口服黄芪多糖能显著促进小鼠淋巴细胞对刀豆蛋白A(ConA)和脂多糖(LPS)的刺激反应,并增加十二指肠IELs和固有层IgA+细胞数量;口服黄芪多糖后接种FMD疫苗,能够显著增强血清FMD特异性IgG及其亚类水平。
[Abstract]:Forty mice were randomly divided into two groups: one group was treated with astragalus polysaccharide solution and the other group was treated with normal saline as control group. Five mice were randomly selected from each group every week after administration, and spleen lymphocytes were isolated for lymphocyte proliferation test. The number of IgA cells in intraepithelial lymphocytes and lamina propria of duodenal mucosa were observed, 24 mice were randomly divided into 4 groups, each mice were divided into 2 groups and injected with foot-and-mouth disease vaccine at intervals of 2 weeks. The mice in group 1 and group 3 received different doses of astragalus polysaccharide before immunization, and mice in group 4 were treated with normal saline as control. Blood samples were collected for 5 weeks after the second immunization. Serum IgG and its subclasses were detected. Astragalus polysaccharides could significantly promote the stimulation of lymphocytes to concanavalin Cona and lipopolysaccharide (LPS), and increase the number of duodenal IELs and lamina propria IgA cells. Astragalus polysaccharide was given to FMD vaccine after oral administration of Astragalus polysaccharide. Serum FMD specific IgG and its subclasses were significantly enhanced.
【作者单位】：浙江大学动物科学学院;贵州省畜牧兽医学校;
【基金】：公益性行业(农业)科研专项资助项目(201303040-03)
【分类号】：S855.3

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