肠炎沙门菌感染对济宁百日鸡盲肠TLR2、TLR4、NOD1、NALP3基因表达的影响

发布时间：2018-03-02 13:45

本文选题：济宁百日鸡　切入点：肠炎沙门菌　出处：《中国家禽》2017年08期 　论文类型：期刊论文

【摘要】：为分析肠炎沙门菌感染后不同时间点济宁百日鸡盲肠TLR2、TLR4、NOD1和NALP3基因表达的变化规律,试验选用2日龄肠炎沙门菌阴性济宁百日鸡为试验动物,接种肠炎沙门菌,应用荧光定量PCR分别检测接种后第1、3、7、14、21、28和35天试验组与对照组盲肠组织TLR2、TLR4、NOD1、NALP3 4个基因的表达变化。结果:肠炎沙门菌感染后第3、7、21、28和35天,试验组和对照组盲肠TLR2的表达量差异显著(P0.05),除感染后第3天TLR2的表达量显著降低外,其他各时间点TLR2的表达量均显著升高(P0.05);感染后第3、21、28天TLR4的表达量显著升高,分别为对照组的1.79、1.52、1.21倍(P0.05);感染后第7天,试验组NOD1和NALP3的表达量均显著低于对照组,分别为对照组的0.37和0.61倍(P0.05);感染后第21天,试验组NALP3基因的表达量显著升高,为对照组的1.27倍(P0.05)。研究结果表明:TLRs和NLRs基因在肠炎沙门菌感染后被激活,且表现出协同调控作用,这种协同调控在感染后第7天最为明显。
[Abstract]:In order to analyze the changes of TLR2TLR4 NOD1 and NALP3 gene expression in the cecum of Chicken at different time points after Salmonella enteritis infection, salmonella enteritidis negative Chicken was selected as experimental animal and Salmonella enteritidis was inoculated with Salmonella enteritidis. Fluorescence quantitative PCR was used to detect the expression of four genes of TLR2TLR4NOD1 and NALP3 in the caecum of the experimental group and the control group on the 1st and 35th day after inoculation. Results: Salmonella enteritis infected Salmonella enteritidis on the 2nd and 35th day. There was a significant difference in the expression of TLR2 between the experimental group and the control group (P 0.05). Except for the decrease of TLR2 expression on the 3rd day after infection, the expression of TLR2 increased significantly at all other time points, and the expression of TLR4 increased significantly on the 28th day after infection. On the 7th day after infection, the expression of NOD1 and NALP3 in the experimental group was significantly lower than that in the control group, 0.37 and 0.61 times of the control group, respectively, and the expression of NALP3 gene in the experimental group increased significantly on the 21st day after infection. The results showed that the genes of 1: TLRs and NLRs were activated after Salmonella enteritis infection and showed synergistic regulation, which was most obvious on the 7th day after infection.
【作者单位】：山东农业大学动物科技学院;山东农业大学生命科学学院;
【基金】：国家自然科学基金项目(31601980) 山东省现代农业产业技术体系项目(SDAIT-11-02) 山东省农业良种工程项目
【分类号】：S858.31

【相似文献】