利用RNA-seq技术筛选大白猪皮下和肌内脂肪组织差异表达基因

发布时间：2018-03-02 23:37

本文选题：肌内脂肪　切入点：皮下脂肪　出处：《遗传》2017年06期 　论文类型：期刊论文

【摘要】：为了比较分析大白猪皮下和肌内脂肪组织的全转录组数据,探究调控脂肪沉积的分子机制,本文采用RNA-seq技术和生物信息学方法鉴定大白猪皮下和肌内脂肪组织基因表达谱,对差异表达基因进行GO(Gene Ontology)分析、信号通路富集分析以及蛋白互作网络分析。大白猪皮下和肌内脂肪组织中有180个基因差异表达,上调基因主要参与细胞增殖、脂质激酶活性和磷脂代谢等与脂质代谢相关的生物学过程正调控,下调基因显著富集于脂肪细胞分化中起重要调控作用的MAPK信号转导通路。差异表达基因主要通过参与脂质代谢及通过MAPK信号转导通路调控脂肪细胞成脂分化,进而影响大白猪皮下和肌内脂肪的沉积。
[Abstract]:In order to compare and analyze the total transcriptional data of subcutaneous and intramuscular adipose tissue of white pig and to explore the molecular mechanism of regulating fat deposition, RNA-seq technique and bioinformatics were used to identify the gene expression profiles of subcutaneous and intramuscular adipose tissue in white pig. The differentially expressed genes were analyzed by GO(Gene Ontology analysis, signal pathway enrichment analysis and protein interaction network analysis. 180 genes were differentially expressed in subcutaneous and intramuscular adipose tissues of white pigs, and up-regulated genes were mainly involved in cell proliferation. The biological processes related to lipid metabolism, such as lipid kinase activity and phospholipid metabolism, are positively regulated. The down-regulated genes are significantly enriched in the MAPK signal transduction pathway, which plays an important role in adipocyte differentiation. The differentially expressed genes are mainly involved in lipid metabolism and adipocyte adipogenic differentiation through MAPK signal transduction pathway. In turn, it affects the deposition of subcutaneous and intramuscular fat in white pigs.
【作者单位】：中国农业科学院北京畜牧兽医研究所;
【基金】：中央级公益性科研院所基本科研业务费专项资金项目(编号:2013ywf-yb-5,2013ywf-zd-2) 中国农业科学院农业科技创新项目(编号:ASTIP-IAS05);中国农业科学院创新基金项目(2004-院-1) 国家高技术研究发展计划项目(“863”计划)(编号:2008AA10Z140) 国家重点基础研究发展计划项目(“973”计划)(编号:2015CB943100)资助~~
【分类号】：S828

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