犬血型DEA1.1抗原生化特性的研究

发布时间：2018-03-03 08:30

本文选题：犬　切入点：血型　出处：《黑龙江畜牧兽医》2017年19期 　论文类型：期刊论文

【摘要】：为了研究犬血型DEA1.1抗原生化特性,为犬输血前血型检测提供理论依据,试验采用凝聚胺介质试剂调查356只不同地区及品种犬的DEA1.1血型频率分布,用考马斯亮蓝法检测DEA1.1红细胞膜蛋白并通过免疫沉淀鉴定血型蛋白大小,观察多种酶处理对DEA1.1抗原性的影响,通过ABO血型检测试剂和ABO型微柱凝胶检测卡检测人类红细胞ABO血型与犬DEA1.1血型有无交叉反应。结果表明:不同品种犬的血型频率差异较大,DEA1.1血型为50 ku和200 ku膜蛋白;DEA1.1抗原在无花果酶、木瓜酶、链霉蛋白酶处理后抗原性增强,在菠萝酶、糜蛋白酶和胰蛋白酶处理后抗原性未增强;DEA1.1阴、阳性红细胞与ABO血型试剂均未发生凝集。说明为降低输血反应,最好采用DEA1.1血型频率低的犬种作为供血犬,并建议输血前采用木瓜酶介质交叉配血试验进行血型检测。
[Abstract]:In order to study the biochemical characteristics of canine blood group DEA1.1 antigen and provide theoretical basis for blood group detection before blood transfusion, the frequency distribution of DEA1.1 blood group in 356 dogs from different regions and breeds was investigated by polyacrylamide medium reagent. The erythrocyte membrane protein of DEA1.1 was detected by Coomassie brilliant blue method and the size of blood group protein was identified by immunoprecipitation. The effects of various enzyme treatments on the antigenicity of DEA1.1 were observed. ABO blood group detection reagent and ABO microcolumn gel test card were used to detect the cross reaction between human red blood cell ABO blood group and canine DEA1.1 blood group. The results showed that the frequency of DEA1.1 blood group was 50 ku and 200ku membrane. Protein DEA1.1 antigens in figs, The antigenicity of papain and strepsin was enhanced, but the antigenicity of Pineapple, chymotrypsin and trypsin was not enhanced after treatment with DEA1.1 negative, the agglutination of positive erythrocytes and ABO blood group reagent was not observed. The dogs with low DEA1.1 blood group frequency should be used as blood supplying dogs, and the papain enzyme medium cross matching test should be used to detect blood type before blood transfusion.
【作者单位】：青岛农业大学动物科技学院;
【基金】：国家自然科学基金项目(31572590;31502138) 山东省自然科学基金项目(BS2015NY001) 山东省高等学校科技计划项目(J15LF03)
【分类号】：S858.292

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