稀释和低温保存对鸡精液品质和受精能力的影响

发布时间：2018-03-03 11:03

  本文选题：鸡　切入点：精液　出处：《畜牧兽医学报》2017年04期 　论文类型：期刊论文

【摘要】：本研究旨在探索稀释和低温保存处理对鸡精液品质和受精能力的影响,以优化鸡精液的短期保存条件。试验采集30只北京油鸡精液,混匀后等量分为4组:A组精液不处理作为对照,B组用BPSE稀释液按照1∶1比例稀释,C组精液离心后,去除上层精浆,加入与精浆等体积的BPSE稀释液重新混匀,D组在C组处理的基础上再用BPSE稀释液按照1∶1比例稀释。每组精液分为两份,一份用于直接输精和精液品质测定,另一份在4℃保存,期间每隔8h检测一次精液品质,24h后输精,每组收集种蛋120枚左右,统计受精率和孵化率等指标。结果表明:不同稀释处理后,C和D组精子活力极显著低于A组对照和B组(P0.01),B组和C组的受精率、入孵蛋的孵化率和受精蛋的孵化率与A组无显著差异,但是D组3项指标均极显著低于A组对照(P0.01)。随着4℃保存时间的延长,4组精子活力均极显著降低(P0.01),其中C和D组降低的幅度显著低于A组对照和B组。精子畸形率随着低温保存时间的延长而显著增加(P0.05),且畸形主要表现为头部肿胀,颈部弯曲和尾部断裂。与直接输精相比,低温保存24h后,A、B和C组的受精率和孵化率都显著降低(P0.05),但是D组低温保存前后的受精率和孵化率差异均不显著(P0.05),分别可以达到71%和82%。综上表明,新鲜精液可以进行1∶1稀释后输精,在不影响受精率的前提下可以提高公鸡配种能力。在需要短期保存精液后进行输精时,可以将采集的新鲜精液离心去除精浆并进行稀释,保存于4℃,从而降低精子活力的下降幅度,维持受精能力。
[Abstract]:The purpose of this study was to explore the effects of dilution and cryopreservation on the quality and fertilization ability of chicken semen in order to optimize the short-term preservation conditions of chicken semen. After blending, the semen of group B was divided into 4 groups: group A, without treatment, and group B, with BPSE diluting the semen of group C according to the ratio of 1: 1, after centrifugation, the upper seminal plasma was removed. Group D was remixed with seminal plasma of equal volume of BPSE diluent and then diluted with BPSE diluent in proportion of 1: 1 on the basis of group C. Each group of semen was divided into two parts, one for direct insemination and determination of semen quality, the other for preservation at 4 鈩，

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