结核分枝杆菌耐药机制与检测技术研究进展

发布时间：2018-03-03 12:21

本文选题：结核分枝杆菌　切入点：耐药性检测　出处：《中国畜牧兽医》2017年06期 　论文类型：期刊论文

【摘要】：近年来耐药结核分枝杆菌的不断出现给结核病的治疗带来前所未有的压力。研究发现,不但牛型分枝杆菌可感染人,人型结核分枝杆菌也可感染牛,这也给养牛业的发展带来了威胁与挑战。如何建立快速、简便、敏感性强、特异性好、价格低廉的耐药性检测方法是控制该病蔓延的关键。作者阐述了常用药物异烟肼、利福平、链霉素、乙胺丁醇、吡嗪酰胺等的作用机制及传统分枝杆菌耐药性检测方法(荧光素酶系统、显微镜观察药敏检测技术(MODS)、比例法、Bactec MGIT 960系统)和以分子生物学为基础的分枝杆菌耐药性检测方法(单链探针反向杂交试验(LiPA)、Gene Xpert全自动检测系统、PCR-单链构象多态性分析(PCR-SSCP)、PCR-限制性片段长度多态性分析(PCR-RFLP)、DNA测序、基因芯片技术)的研究进展,以期为进一步的研究与应用提供参考。
[Abstract]:In recent years, the emergence of drug-resistant Mycobacterium tuberculosis has brought unprecedented pressure to the treatment of tuberculosis. Studies have found that not only can Mycobacterium bovis infect human beings, but also human Mycobacterium tuberculosis can infect cattle. This has also brought threats and challenges to the development of the cattle industry. The key to control the spread of the disease is to establish a rapid, simple, sensitive, specific and inexpensive method for the detection of drug resistance. The mechanisms of rifampicin, streptomycin, ethambutanol, pyrazinamide, etc., and traditional methods for detection of mycobacterium resistance (luciferase system, luciferase system, etc.). Microscopical observation of drug sensitivity detection techniques: MODSX, proportional method, Bactec MGIT 960 system, and molecular biology-based detection methods for drug resistance of Mycobacteria (single strand probe reverse hybridization test) Gene Gene Xpert automated detection system for PCR- single strand conformation polymorphism. PCR-SSCP- restriction fragment length Polymorphism (PCR-RFLPU) DNA sequencing. In order to provide reference for further research and application.
【作者单位】：中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部草食动物疫病重点开放实验室;
【基金】：国家自然科学基金(31172335)
【分类号】：S852.618

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