新疆部分地区牛源空肠弯曲菌分离鉴定及耐药性分析

发布时间：2018-03-03 14:40

本文选题：空肠弯曲菌　切入点：PCR　出处：《新疆农业科学》2017年09期 　论文类型：期刊论文

【摘要】：【目的】研究新疆部分地区牛空肠弯曲菌污染及耐药性。【方法】从乌鲁木齐、昌吉、石河子3个地区共采集牛肛拭子171份。用布氏肉汤初步增菌后,用改良CCD琼脂基础和哥伦比亚血平板2种选择培养基培养,用聚合酶链式反应(PCR)鉴定,最后参照2010年美国临床实验室标准化研究所(CLSI)推荐使用的琼脂扩散法进行药敏试验。【结果】从乌鲁木齐牛场肛拭子分离鉴定到1株空肠弯曲菌(编号为N22),检出率为1.11%(1/90);昌吉牛场肛拭子分离鉴定到1株空肠弯曲菌(编号为C25),检出率为3.33%(1/30)。石河子牛场肛拭子没有分离到空肠弯曲菌。2株空肠弯曲菌对单环β内酰胺类、氨基糖苷类抗生素高度敏感;对喹诺酮类抗菌药物、大环内酯类和大部分头孢菌素类抗生素产生了不同程度的耐药。【结论】新疆牛群存在空肠弯曲菌的感染,感染菌株可用单环β-内酰胺类、氨基糖苷类抗生素进行防治。研究结果可为评价新疆牛群空肠弯曲菌的流行状况和制定防控措施提供科学依据。
[Abstract]:[objective] to study the contamination and drug resistance of Campylobacter jejuni in Xinjiang. [methods] 171 samples of bovine anal swabs were collected from Urumqi, Changji and Shihezi areas. Two kinds of selective culture medium, modified CCD Agar foundation and Colombian blood plate, were used for identification, and were identified by polymerase chain reaction (PCR). Finally, according to the Agar diffusion method recommended by American Institute of Clinical Laboratory Standardization in 2010, a strain of Campylobacter jejuni was isolated and identified from anal swab of Urumqi cattle farm. A strain of Campylobacter jejuni (C25) was isolated from the anal swab of Changji cattle farm, and the detection rate was 3.33 ~ 1 / 30 ~ 0.No 2 strains of Campylobacter jejuni were isolated from the anal swab of Shihezi cattle farm. Aminoglycoside antibiotics are highly sensitive to quinolones, macrolides and most cephalosporins. [conclusion] there is an infection of Campylobacter jejuni in cattle in Xinjiang. The infection strains can be prevented and controlled by monocyclic 尾 -lactams and aminoglycoside antibiotics. The results can provide scientific basis for evaluating the epidemic situation of Campylobacter jejuni in Xinjiang cattle and for formulating prevention and control measures.
【作者单位】：新疆农业大学动物医学学院;
【基金】：新疆维吾尔自治区自然科学基金项目“新疆牛源弯曲菌污染分布规律及分离株遗传多样性研究”(2015211A021)~~
【分类号】：S852.61

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