不同日龄略阳乌鸡肌肉中肌苷酸含量的变化规律

发布时间：2018-03-04 12:16

本文选题：略阳乌鸡　切入点：肉品质　出处：《江苏农业科学》2016年12期 　论文类型：期刊论文

【摘要】：以18日胚龄及6、180日龄黄羽鸡和略阳乌鸡为研究对象,探讨不同发育时期肌肉中风味物质的形成规律。采用高效液相色谱仪测定腿肌和胸肌中的肌苷酸含量,利用荧光定量PCR方法检测肌苷酸合成关键酶腺苷琥珀酸裂解酶(ADSL)基因的mRNA表达情况。结果显示:黄羽鸡和略阳乌鸡腿肌中ADSL基因的表达、肌苷酸的含量均在6日龄时最高,胸肌中肌苷酸在180日龄时含量最高,表达趋势基本一致;略阳乌鸡腿肌和胸肌的肌苷酸含量在180日龄时明显高于黄羽鸡。同时,ADSL基因的表达量在不同时期均明显高于黄羽鸡,表明成年略阳乌鸡的肉品质优于黄羽鸡。
[Abstract]:In 18th and 6,180 days old yellow feather chicken and Lueyang silky chicken were used to study the formation of flavor substances in muscle of different developmental stages. The content of inosine in leg muscle and pectoral muscle was determined by high performance liquid chromatograph (HPLC), and the content of inosine in leg muscle and breast muscle was determined by high performance liquid chromatograph (HPLC). The mRNA expression of adenosine succinate lyase (ADSL) gene, a key enzyme of inosine synthesis, was detected by fluorescence quantitative PCR. The results showed that the expression of ADSL gene in leg muscle of yellow feather chicken and Lueyang silky chicken was the highest at the age of 6 days. The content of inosine in pectoralis muscle was the highest at 180 days of age and the expression trend was basically the same. The content of inosine in leg muscle and breast muscle of Lueyang silky chicken was significantly higher than that of yellow feather chicken at 180 days old, and the expression of ADSL gene was significantly higher than that of yellow feather chicken at different stages. The results showed that the meat quality of adult Lueyang silky chicken was better than that of yellow feather chicken.
【作者单位】：陕西理工学院生物科学与工程学院;
【基金】：陕西省科技厅农业攻关项目(编号:2013K02-26-02) 陕西省教育厅项目(编号:12JS028)
【分类号】：S831.1

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