某集约化猪场猪瘟、蓝耳病抗体水平监测及免疫程序分析和完善

发布时间：2018-03-05 20:48

本文选题：猪瘟　切入点：猪繁殖与呼吸障碍综合征　出处：《上海交通大学》2015年硕士论文　论文类型：学位论文

【摘要】：猪瘟(Classical swine fever,CSF)、猪繁殖与呼吸障碍综合征(Porcine reproductive and respiratory syndrome,PRRS)是全世界养猪业危害较大的两大疫病。本研究选取大型猪场内猪群作为研究对象,进行猪场定期CSF和PRRS血清抗体水平监测,并通过不同的免疫途径,检测抗体效价结果,确定更合适的免疫程序,以期为疫病的防控和流行病学调查提供科学依据。本研究于2013年-2014年对某大型猪场血清用CSF和PRRS相应的抗体或抗原检测试剂盒检测分析了两个不同免疫程序下的猪群免疫抗体水平和感染情况。2014年3月前CSF按6周龄首免,8周龄二免,28周龄三免。此阶段,我们每个月随即采取4周龄仔猪、8周龄和11周龄仔猪血液各30份进行CSF抗原抗体检测,共检测了5次,结果4周龄仔猪猪瘟母源抗体平均阳性率和抗原平均阳性率分别为36.00%和6.67%,8周龄和11周龄仔猪猪瘟抗体平均阳性率和猪瘟抗原平均阳性率分别为47.03%、60.67%和1.33%、0%;2014年3月后调整免疫将首免提前至4周龄,7周龄二免,28周龄三免。此阶段,我们每个月随即采取免疫前4周龄仔猪、6周龄和10周龄仔猪血液各30份进行CSF抗原抗体检测,共检测了5次,结果4周龄仔猪猪瘟母源抗体平均阳性率和抗原平均阳性率分别为36.66%和0.67%,6周龄和10周龄仔猪猪瘟抗体平均阳性率和猪瘟抗原平均阳性率分别为62.00%、70.67%和0%、0%。统计学分析表明,两种免疫效果组间差异显著,其中,4周龄的血清检测两种免疫程序差异不显著(P0.05),而其他两个阶段的检测结果,4周龄首免要优于6周龄首免(P0.05),说明猪瘟疫苗首免选择在仔猪28日龄左右效果好。同时PRRS采用两种免疫程序进行免疫效果试验,4周龄首免PRRS疫苗的仔猪,15周龄和33周龄PRRS抗体平均阳性率分别为85.33%和91.33%;1周龄首免PRRS疫苗的仔猪,15周龄和33周龄时PRRS抗体平均阳性率分别为94.67%和98.00%。两者差异不显著(P0.05),该猪场PRRS疫苗免疫效果比较理想。
[Abstract]:Classical swine fever (Classical swine fever, CSF), porcine reproductive and respiratory syndrome (Porcine reproductive and respiratory syndrome, PRRS) is the world's two major diseases in pig industry harm is greater. This study selected large farms pigs as the research object, to set the CSF and pig serum antibody level of PRRS monitoring, and through immunization the detection of antibody titer results, determine the appropriate immunization program, to provide a scientific basis for the prevention and control of epidemic disease and epidemiological investigation. The research on 2013 -2014 years of a large pig serum CSF and PRRS with the corresponding antibody or antigen detection kit for detection and analysis of two different immune procedures under the swine antibody and the level of.2014 infection in March before the CSF according to the age of 6 weeks from the first 8 weeks of age, two free, 28 weeks of age three free. In this period, every month we took 4 week old piglets, 8 weeks and 11 weeks The blood 30 copies of detection of CSF antigen and antibody were detected, 5 times, the average positive rate of 4 week old piglets swine fever maternal antibody antigen and the average positive rate were 36% and 6.67%, the average positive rate of 8 week old and 11 week old piglets swine fever antibody and the average positive rate of swine fever antigen respectively 47.03%, 60.67% and 1.33%, 0%; after March 2014 will be the first free adjustment of the immune advance to 4 weeks, 7 weeks and two free, 28 week old three free. At this stage, we take each month immediately before immunization of piglets with 4 weeks, 6 weeks and 10 week old piglets were detected 30 CSF antigen antibodies were detected in 5 times, the average positive rate of 4 week old piglets swine fever maternal antibody antigen and the average positive rate were 36.66% and 0.67%, the average positive rate of 6 week old and 10 week old piglets swine fever antibody and the average positive rate of swine fever antigen were 62%, 70.67% and 0%, 0%. statistical analysis table Ming, two free The difference between the groups was plague, the serum of 4 week old two immunization program had no significant difference (P0.05), while the other two stages of the test results, the first 4 weeks than 6 weeks of age the first free free (P0.05), that the first free choice of swine fever vaccine in piglets of 28 days effect at the same time. PRRS uses two kinds of immunization program were immune effect test, 4 week old the first free PRRS vaccine of piglets, the average positive rate of 15 and 33 weeks old PRRS antibody were 85.33% and 91.33%; 1 weeks old the first free PRRS vaccine of piglets, 15 and 33 weeks of age the average positive rate of PRRS both antibodies were 94.67% and 98.00%., the difference was not significant (P0.05), the pig PRRS vaccine effect is ideal.

【学位授予单位】：上海交通大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.28

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