骆驼肠道内3种含氮杂环化合物富集培养的微生物多样性

发布时间：2018-03-07 23:29

本文选题：消化道　切入点：骆驼　出处：《厦门大学学报(自然科学版)》2017年04期 　论文类型：期刊论文

【摘要】：为研究骆驼(Camelus bactrianus)体内是否存在潜在的杂环化合物降解菌,采集3头骆驼肠道内容物,分别以吡啶、喹啉和吲哚3种含氮杂环化合物作为唯一碳源和氮源进行5代富集培养.通过高通量测序技术对肠道内容物和5代富集培养微生物进行了测序分析.结果显示:骆驼肠道中经过杂环化合物(吡啶、喹啉、吲哚)富集后,变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、浮霉菌门(Planctomycetes)、拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes)5个门类在富集的微生物中丰度最高;骆驼肠道内吡啶富集的优势菌属于芽孢杆菌属(Bacillus)、鞘氨醇杆菌属(Sphingo-bacterium)、苍白杆菌属(Ochrobactrum)和假单胞菌属(Pseudomonas),吲哚富集的优势菌属于芽孢杆菌属、棒状杆菌属(Corynebacterium)和赖氨酸芽孢杆菌属(Lysinibacillus),而喹啉富集的优势菌以鞘氨醇杆菌属为主.骆驼肠道内容物原始样品经过吡啶、喹啉、吲哚富集5代后,优势菌群发生了较大的改变,这暗示骆驼肠道内存在具有降解吡啶、吲哚和喹啉潜能的微生物,且涉及这3种杂环化合物的降解菌群不同.
[Abstract]:In order to study the existence of potential heterocyclic compound degrading bacteria in Camelus bactrianus, three camel intestinal contents were collected and used as pyridine. Three nitrogen-containing heterocyclic compounds, quinoline and indole, were enriched and cultured for 5 generations as the sole carbon and nitrogen sources. The intestinal contents and 5 generation enriched microbes were sequenced and analyzed by high throughput sequencing. The results showed that: 1. The passage of heterocyclic compounds (pyridine, pyridine) in camel intestines. After enrichment of quinoline and indole, Proteobacteria, Actinobacteria, Planctomycetesus, Bacteroidetesand Firmicutes of Proteobacteria, Actinobacteria, Planctomycetesus, Bacteroidetesand Firmicutes. were the most abundant in the enriched microorganism. The dominant bacteria for pyridine enrichment in camel intestine belong to Bacillus, Sphingo-bacillus, Ochrobactround and Pseudomonas, and Bacillus to Bacillus, to Sphingo-bacillus, to Pseudomonas, to Bacillus, to Bacillus, to Bacillus, to Pseudomonas sp., to Pseudomonas pseudomonas to Pseudomonas pallidus, to Bacillus, to Bacillus, to Sphingo-bacillus, to Pseudomonas. Corynebacterium (Corynebacterium) and Lysinibacillus (Lysinibacillus), and the predominant bacteria enriched by quinoline were sphingosine. After five generations of enrichment of the original samples of camel intestinal contents by pyridine, quinoline and indole, the dominant flora changed greatly. This suggests that there are many microorganisms with the potential to degrade pyridine, indole and quinoline in camel intestines, and the degradation flora of these three heterocyclic compounds are different.
【作者单位】：新疆师范大学生命科学学院新疆特殊环境物种保护与调控生物学实验室干旱区植物逆境生物学实验室;乌鲁木齐市职业中等专业学校;成都医学院检验医学院;
【基金】：国家自然科学基金(31160028) 国家自然科学基金委员会-新疆维吾尔自治区人民政府联合基金(U1503101) 四川省教育厅重点项目(17ZA0105) 成都医学院校基金项目(CYZ15-06) 新疆师范大学博士科研启动基金(XJNUBS1607) 新疆维吾尔自治区百名博士引进计划(BS2016008)
【分类号】：Q93;S824

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