外源褪黑素处理方式对荷斯坦奶牛体内褪黑素富集与代谢的影响

发布时间：2018-03-08 12:18

本文选题：荷斯坦奶牛　切入点：褪黑素　出处：《中国畜牧兽医》2017年09期 　论文类型：期刊论文

【摘要】：为研究正常情况下经产荷斯坦奶牛24h内血液中褪黑素浓度的变化情况,探索不同外源褪黑素处理方式对荷斯坦奶牛血液褪黑素浓度的影响,本研究采用颈静脉注射、颈部皮下注射、颈部皮下埋植和口腔灌服外源褪黑素等方式处理经产荷斯坦奶牛,观察血液中褪黑素浓度的变化情况并分析褪黑素在体内的分泌规律及代谢速率。结果显示,在24h内,经产荷斯坦奶牛血液中褪黑素浓度变化明显,04∶00浓度最高,为8.30ng/mL,16∶00浓度最低,为2.08ng/mL。皮下埋植和口腔灌服外源褪黑素以后,血液中褪黑素浓度升高幅度较小;皮下注射46.4、4.64和0.464mg褪黑素后,血液中褪黑素浓度迅速升高,1h后达到峰值,分别为561.94、487.03、92.89ng/mL,极显著高于注射前(P0.01);静脉注射46.4、4.64和0.464mg褪黑素后,血液中褪黑素浓度迅速升高,0.5h之后达到峰值,分别为767.68、639.19、110.56ng/mL,极显著高于注射前(P0.01),高水平褪黑素可稳定持续48h。本研究证明了荷斯坦奶牛体内褪黑素分泌有明显的昼夜节律性,静脉或皮下注射外源褪黑素可以迅速提高血液中褪黑素的浓度,且血液中褪黑素的代谢速度与褪黑素的浓度高度相关,随着血液中褪黑素浓度的增加,褪黑素的代谢速度明显加快。
[Abstract]:In order to study the changes of blood melatonin concentration in Holstein cows under normal conditions and to explore the effects of different exogenous melatonin treatments on the blood melatonin concentration of Holstein cows, the study was carried out by jugular vein injection. The changes of melatonin concentration in blood of Holstein cows were observed and the secretion regularity and metabolic rate of melatonin in vivo were analyzed by the methods of cervical subcutaneous injection, cervical subcutaneous implantation and oral administration of exogenous melatonin. Within 24 hours, the concentration of melatonin in blood of Holstein cows was the highest (8.30ng / mL16: 00), and the lowest was 2.08ng / mL.After subcutaneous implantation and oral administration of exogenous melatonin, the concentration of melatonin in blood increased slightly. After subcutaneous injection of 46.4mg melatonin 4.64 and 0.464mg melatonin, the concentration of melatonin in blood rapidly increased to a peak of 561.94-487.03 渭 g / mL after 1 h, respectively, which was significantly higher than that of P0.01g / ml before injection, and reached the peak after intravenous injection of 46.4mg melatonin and 0.464 mg melatonin, and reached the peak after 0.5 h of rapid increase in the concentration of melatonin in blood, and the concentration of melatonin in blood reached its peak after intravenous injection of 46.4mg melatonin and 0.464mg melatonin, respectively. The results showed that melatonin secretion in Holstein cows had a significant circadian rhythm. Intravenous or subcutaneous injection of exogenous melatonin can rapidly increase the concentration of melatonin in the blood, and the metabolic rate of melatonin in blood is highly correlated with the concentration of melatonin, with the increase of the concentration of melatonin in blood. The metabolic rate of melatonin was accelerated obviously.
【作者单位】：中国农业大学动物科技学院畜禽育种国家工程实验室农业部动物遗传育种与繁殖重点实验室北京市动物遗传改良重点实验室;全国畜牧总站;黑龙江省农垦科学院畜牧兽医研究所;北京奶牛中心;吉林农业大学动物科技学院;华中农业大学;
【基金】：北京市奶牛产业创新团队(BAIC06-2016) 国家自然科学基金项目(31372306)
【分类号】：S823

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