饲粮高铁对肉仔鸡十二指肠黏膜铁转运载体基因表达及组织微量元素含量的影响

发布时间：2018-03-09 22:37

本文选题：铁　切入点：铁转运载体基因表达　出处：《动物营养学报》2017年06期 　论文类型：期刊论文

【摘要】：本试验旨在研究饲粮铁含量对肉仔鸡组织重要微量元素铁、锰、铜、锌含量及十二指肠黏膜主要铁转运载体基因表达的影响,探讨铁对肉仔鸡微量元素吸收和代谢的影响及其机制。将336只1日龄商品代罗斯308肉公雏按照体重随机分成4个组,每组6个重复,每个重复14只鸡。对照组饲喂不额外添加铁的基础饲粮(实测铁含量为78 mg/kg),铁添加组分别饲喂以七水硫酸亚铁(FeSO_4·7H_2O)形式添加100、250或500 mg/kg铁的试验饲粮(实测铁含量分别为166、308和579 mg/kg)。试验期21 d。各组试鸡分别于7、14和21日龄屠宰分析肝脏、心脏、胰腺、十二指肠黏膜和胫骨灰中铁、锰、铜、锌含量及十二指肠黏膜中二价金属转运蛋白(DMT1)和膜铁转运蛋白(FPN1)mRNA表达水平。结果表明:1)500 mg/kg铁添加组1~7日龄和8~14日龄的平均日增重显著低于其他3组(P0.10),250和500 mg/kg铁添加组1~7日龄的平均日采食量显著低于其他2组(P0.10)。2)饲粮铁含量对肉仔鸡7、14、21日龄的血浆总铁结合力以及全血血红蛋白浓度(7日龄除外)和红细胞压积均无显著影响(P0.10),但显著影响7、14和21日龄血浆铁含量和铁饱和度(P0.10),二者均随饲粮铁含量增加而升高。3)7和14日龄心脏及7、14和21日龄肝脏、十二指肠黏膜、胰腺和胫骨灰铁含量均随饲粮铁含量的增加而升高,7、14和21日龄十二指肠黏膜、胰腺和胫骨灰锰含量均随饲粮铁含量的增加而降低;饲粮添加铁显著降低7日龄胰腺锌含量(P0.10),但对其他日龄胰腺和各日龄其他所测组织锌含量以及各日龄所测各组织铜含量均无显著影响(P0.10)。4)饲粮铁含量显著影响7、14和21日龄十二指肠黏膜DMT1和FPN1 mRNA表达水平(P0.10),各日龄DMT1和FPN1mRNA表达水平均随饲粮铁含量的增加而降低。以上结果提示,高铁饲粮可能通过调控十二指肠黏膜DMT1和FPN1基因的表达降低锰和锌在肠道的吸收,进而减少锰和锌在组织中的沉积。
[Abstract]:The purpose of this study was to investigate the effects of dietary iron content on the expression of iron, manganese, copper, zinc and major iron transporter genes in duodenal mucosa of broilers. To investigate the effect of iron on the absorption and metabolism of trace elements in broilers, 336 1-day-old broilers were randomly divided into 4 groups according to their body weight, 6 replicates in each group. The control group was fed a basal diet (the measured iron content was 78 mg / kg 路kg ~ (-1)) and the iron supplementation group was fed with FeSO _ 4 路7H _ 2O in the form of Fe _ 2O _ 4 路7H _ 2O (measured iron content). They were 166,308 mg / kg and 579 mg / kg 路kg ~ (-1), respectively. The liver was slaughtered and analyzed at the age of 7 ~ 14 and 21 days respectively during the trial period of 21 days. Iron, manganese, copper in heart, pancreas, duodenal mucosa and tibial ash, Zinc content, divalent metal transport protein (DMT1) and membrane iron transport protein (FPN 1) mRNA expression in duodenal mucosa. The results showed that the average daily gain (ADG) was significantly lower than that in the other three groups at the age of 7 days and 8 ~ 14 days after addition of 1: 1 ~ 500 mg/kg iron group. The results showed that the average daily gain of the three groups was significantly lower than that of the other three groups (P _ (0.10) O _ (250) and P _ (500) mg/kg). The average daily feed intake of the addition group was significantly lower than that of the other two groups (P 0.10 路2) the total iron binding capacity of plasma and the whole blood hemoglobin concentration and hematocrit were not significantly affected by the dietary iron content in the 7 ~ (14) ~ (21) day old broilers except for the 7 ~ (th) day) and the hematocrit (P _ (0.10)). However, the plasma iron content and iron saturation were significantly affected at 7 ~ 14 and 21 days of age. Both of them increased with the increase of dietary iron content. The heart of 7 and 14 days of age and liver of 7 ~ 14 and 21 days of age were increased with the increase of dietary iron content. The contents of gray iron in duodenal mucosa, pancreas and tibia increased with the increase of dietary iron content. The contents of gray manganese in pancreas and tibia decreased with the increase of dietary iron content. Dietary iron supplementation significantly decreased the zinc content of the pancreas at 7 days of age (P 0.10), but had no significant effect on the zinc content of the other day pancreas and other tissues and copper content of the tissues measured at each day of age. The expression level of DMT1 and FPN1mRNA in duodenal mucosa of 21 days old was P0.10, and the expression level of DMT1 and FPN1mRNA decreased with the increase of dietary iron content. High iron diet may reduce the absorption of manganese and zinc in intestine by regulating the expression of DMT1 and FPN1 genes in duodenal mucosa, and then decrease the deposition of manganese and zinc in tissues.
【作者单位】：河北科技师范学院;中国农业科学院北京畜牧兽医研究所;
【基金】：国家自然科学基金项目(31272465)
【分类号】：S831.5

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