合成绵羊抗菌肽NK-Lysin抗菌活性及其对雏鸡沙门氏菌攻毒的治疗效果研究

发布时间：2018-03-10 22:27

本文选题：绵羊　切入点：抗菌肽　出处：《中国畜牧兽医》2017年09期 　论文类型：期刊论文

【摘要】：为了研究绵羊抗菌肽NK-Lysin主要活性区的生物学功能,试验设计并合成了3对功能区多肽片段,并利用径向扩散试验和最小抑菌浓度对其抗菌活性进行检测,分析了合成多肽对鸡血红细胞的毒性作用,筛选出抗菌效果最好的多肽对沙门氏菌攻毒的雏鸡进行治疗,检测其治疗效果。结果表明,合成多肽对大肠杆菌、沙门氏菌具有抑制活性,多肽片段长度、多肽C-端有无酰胺化及多肽内的二硫键是否成环对多肽的抗菌活性均有影响;筛选出的2个多肽在治疗雏鸡沙门氏菌攻毒的过程中能够明显降低死亡率,对雏鸡心脏、肝脏、肾脏的病理损伤也明显小于对照组。本研究结果为绵羊抗菌肽NK-Lysin作为候选抗菌药物的开发奠定了基础。
[Abstract]:In order to study the biological function of the main active regions of sheep antimicrobial peptide NK-Lysin, three pairs of functional peptide fragments were designed and synthesized, and their antibacterial activities were determined by radial diffusion test and minimal inhibitory concentration. The toxic effect of synthetic polypeptides on chicken blood red blood cells was analyzed. The best antibacterial peptides were selected to treat the chickens infected with Salmonella. The results showed that the synthetic polypeptides were effective on Escherichia coli. Salmonella had inhibitory activity, length of peptide fragment, amidation of C-terminal and cyclization of disulfide bond. The two peptides could significantly reduce the mortality during the treatment of salmonella in chicks, and could significantly reduce the mortality of the heart and liver of chicks. The results of this study laid a foundation for the development of sheep antimicrobial peptide NK-Lysin as a candidate antimicrobial agent.
【作者单位】：石河子大学动物科技学院;
【基金】：国家自然科学基金(31360589) 兵团博士资金专项(2014BB016) 人社部留学回国人员启动(RSLX201304)
【分类号】：S859.79

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