鸭肠炎病毒感染鸭脾脏的转录组分析

发布时间：2018-03-11 00:23

本文选题：鸭肠炎病毒(DEV)　切入点：鸭脾脏　出处：《病毒学报》2017年03期 　论文类型：期刊论文

【摘要】：为探明鸭肠炎病毒(Duck enteritis virus,DEV)感染鸭脾脏的转录组情况,本研究以DEV GZ株经腿部肌肉接种50d鸭,于接种后66h采集鸭脾组织样本,提取总RNA,采用Illumina HiSeq 2000TM对试验组和对照组样品进行测序,筛选DEV感染鸭脾脏组织的差异表达基因,对其进行生物信息学GO功能分类和KEGG信号通路分析。结果显示,差异表达基因共511个,其中表达上调312个,表达下调199个。GO功能分类结果显示,差异表达基因主要涉及氧运输、整合素活化、补体激活等生物学过程,胞外区、血红蛋白复合物、细胞外间隙等细胞组分,氧转运活动、氧结合、核糖体的结构成分等分子功能。KEGG分析表明这些差异表达基因参与ECM受体相互作用、核糖体、CAMs、JAK-STAT信号通路、细胞因子和细胞因子受体相互作用、PPAR信号通路、神经活性配体/受体相互作用信号通路。本研究为深入探究DEV与鸭脾脏组织互作、宿主抗病机制及相关功能基因的筛选奠定了基础。
[Abstract]:In order to find out the transcriptional status of duck spleen infected with duck enteritis virus (Duck enteritis virus), DEV GZ strain was inoculated with leg muscle for 50 d, and the samples of duck spleen tissue were collected at 66 h after inoculation. Total RNAs were extracted, Illumina HiSeq 2000TM was used to sequence the samples of the experimental group and the control group, and the differentially expressed genes in the spleen tissue of the infected duck were screened. The differential expression genes were classified by bioinformatics go and analyzed by KEGG signal pathway. There were 511 differentially expressed genes, including 312 up-regulated genes, 199 down-regulated .go functional classification results showed that differentially expressed genes mainly involved in oxygen transport, integrin activation, complement activation and other biological processes, extracellular domain, etc. Molecular functions of hemoglobin complex, extracellular intercellular components, oxygen transport, oxygen binding, ribosomal structural components, etc. KEGG analysis showed that these differentially expressed genes were involved in the interaction of ECM receptor, and ribosomal CAMsJAK-STAT signaling pathway. The interaction between cytokine and cytokine receptor is the signal pathway of PPAR and the signal pathway of neuroactive ligand / receptor interaction. The purpose of this study was to explore the interaction between DEV and duck spleen. The screening of host disease resistance mechanism and related functional genes laid the foundation.
【作者单位】：贵州大学动物科学学院;贵州大学动物疫病研究所;贵州省动物疫病与兽医公共卫生重点实验室;
【基金】：国家自然科学基金(项目号:31260607),题目:PKCI及PKC在鸭肠炎病毒增殖中的作用研究;国家自然科学基金(项目号:31560703),题目:NF-κB信号通路调控鸭肠炎病毒增殖过程的分子机制研究贵州省优秀青年科学人才培养计划项目(项目号:黔科合人字[2013]25号) 贵州省百层次创新型人才项目(项目号:黔科合人才[2016]4009号) 贵州省科技创新人才团队建设项目(项目号:黔科合人才团队[2015]4016号)~~
【分类号】：S858.32

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