不同发育阶段的BX-SPF鸡消化道内分泌细胞的研究

发布时间：2018-03-11 04:40

本文选题：BX-SPF鸡　切入点：个体发生　出处：《哈尔滨师范大学》2015年硕士论文　论文类型：学位论文

【摘要】：本文应用Grimelius法对BX-SPF鸡11个发育阶段(1天、3天、1周、2周、3周、4周、10周、21周、26周、40周、69周)的消化道嗜银细胞进行了研究。结果显示,以上各发育阶段鸡消化道除食管外,其它部位均有嗜银细胞分布,且细胞形态各异:有圆形、椭圆形、水滴形、锥体形和梭形等,嗜银细胞分布在胃部腺叶区,肠上皮细胞之间、腺泡上皮之间及固有膜内;对鸡胃肠道同一部位不同发育阶段嗜银细胞的分布密度进行多重比较,发现4周龄组胃肠道除了回肠以外,剩余部位均高于其它年龄组,且差异显著;同一发育阶段胃肠道不同部位嗜银细胞相比,各年龄组腺胃和直肠均高于胃肠道其它部位,且差异极显著。本文还应用免疫组织化学ABC法(Avidin-biotin complex method,ABC)对以上各年龄段BX-SPF鸡消化道5-羟色胺(5-Hydroxytryptamine,5-HT)细胞和胃泌素(Gastrin,Gas)细胞进行免疫组织化学鉴别和定位。结果显示,5-HT细胞在各个发育阶段鸡消化道中除食管外,其它部位均有分布(腺胃、小肠、直肠和盲肠);Gas细胞只存在于各年龄中的小肠(十二指肠、空肠和回肠)部位。2种内分泌细胞形态相似,均有圆形、椭圆形、水滴形、锥体形和少量的梭形等,但分布位置有差异,5-HT细胞在胃部主要分布于腺叶区,在肠部主要分布于上皮细胞之间、腺泡上皮之间及固有膜内;而胃泌素细胞主要分布于肠上皮细胞之间、固有膜内,少数分布在腺泡上皮之间偶见。对鸡消化道同一部位不同发育阶段的5-HT细胞及Gas细胞分别进行多重比较,发现26周龄BX-SPF鸡胃肠道5-HT细胞与其它年龄段相比除了直肠外,均高于其它年龄组,且有显著差异,Gas细胞分布密度呈现波浪形变化。5-HT细胞在同一发育阶段胃肠道不同部位之间相比,腺胃部分布密度明显低于胃肠其它部位,而直肠部密度分布又明显高于胃肠其它部位,且差异显著;Gas细胞在同一年龄组小肠不同部位之间相比,回肠部均高于十二指肠和空肠(除1天、3天和1周组外)。以上不同发育阶段消化道内分泌细胞形态、位置以及密度分布特征与鸡出生后各个不同时期生长发育规律以及消化道不同部位生理功能是相适应的。
[Abstract]:In this paper, Grimelius method was used to study the silver eosinophils in the digestive tract of BX-SPF chickens in 11 developmental stages (1 day, 3 days, 1 week, 2 weeks, 4 weeks, 10 weeks, 21 weeks, 26 weeks, 40 weeks, 40 weeks, 69 weeks). The results showed that the digestive tract of the above developed chickens was in addition to the esophagus. In other parts, the distribution of argyrophilic cells was different: round, oval, water droplet, pyramidal and fusiform, and the silver cells were distributed in the glandular lobes of the stomach, between the intestinal epithelial cells, between the acinar epithelium and in the lamina propria. The distribution density of argyrophilic cells in the same part of the gastrointestinal tract in different stages of development was compared. The results showed that the distribution density of the silver eosinophils in the gastrointestinal tract of 4 weeks old was higher than that in the other age groups except the ileum, and the difference was significant. The adenogastric and rectum cells in different parts of the gastrointestinal tract in the same developmental stage were higher than those in the other parts of the gastrointestinal tract. The immunohistochemical identification and localization of 5-hydroxytryptamine 5-HT cells and gastrin gastrin gastrin gas cells in digestive tract of BX-SPF chickens of all ages were carried out by using the immunohistochemical ABC method Avidin-biotin complex method. The results showed that 5-HT cells were located in the digestive tract. In the digestive tract, except esophagus, There were only 2 endocrine cells in the small intestine (duodenum, jejunum and ileum) in all the other sites (glandular stomach, small intestine, rectum and cecum), all of which were round, oval, and water droplet shape, but only in the small intestine (duodenum, jejunum and ileum). The distribution of 5-HT cells was mainly in the glandular lobes in the stomach, between the epithelial cells in the intestine, between the epithelial cells in the acinar epithelium and in the lamina propria. However, gastrin cells were mainly distributed between intestinal epithelial cells, in the lamina propria and a few in acinar epithelium. The 5-HT cells and Gas cells in the same part of the digestive tract were compared in different stages of development. It was found that 5-HT cells in gastrointestinal tract of 26 week old BX-SPF chickens were higher than those of other age groups except rectum. There was significant difference in the distribution density of Gas cells. The distribution density of 5-HT cells in the glandular stomach was significantly lower than that in the other parts of the gastrointestinal tract in the same developmental stage. The density distribution of rectum was significantly higher than that of other parts of gastrointestinal tract, and the difference was significant between different parts of small intestine in the same age group. The ileum was higher than duodenum and jejunum (except 1 day 3 days and 1 week group). The distribution characteristics of position and density were suitable for the growth and development of chicken at different stages after birth and the physiological function of different parts of digestive tract.
【学位授予单位】：哈尔滨师范大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S831

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