屎肠球菌NCIMB11181对大肠杆菌O78感染肉鸡生产性能、肠道微生物和血液抗氧化功能的影响

发布时间：2018-03-11 21:37

本文选题：肉鸡　切入点：屎肠球菌　出处：《中国家禽》2017年11期 　论文类型：期刊论文

【摘要】：试验旨在研究饲粮添加屎肠球菌对禽大肠杆菌O78感染肉鸡生产性能、肠道微生物菌群和血液抗氧化功能的影响。选取288只1日龄罗斯308母鸡,随机分成4组。分别在各试验组基础饲粮中添加0、100、200、300 mg/kg屎肠球菌。各试验组在11日龄均气囊接种禽大肠杆菌,0.2 mL/只。结果表明:与无添加组相比,(1)饲粮中添加屎肠球菌可线性提高10日龄肉鸡体重(BW)(P0.05),线性及二次线性提高22和35日龄肉鸡BW(P0.05),线性提高1~10、10~22和10~35日龄肉鸡平均日增重(P0.05),降低感染后肉鸡死亡率。(2)饲粮中添加屎肠球菌可线性降低感染后3 d盲肠大肠杆菌和沙门菌数量(P0.05),线性及二次线性降低双歧杆菌数量(P0.05);感染后7 d,屎肠球菌添加组大肠杆菌和沙门菌数量线性降低(P0.05)。(3)感染后3和7 d,屎肠球菌组血清总抗氧化能力分别呈线性(P0.05)和二次线性降低(P0.05);血清丙二醛含量均呈线性及二次线性降低(P0.05);谷胱甘肽过氧化物酶活性均为二次线性降低(P0.05)。由此可见饲粮添加屎肠球菌可缓解禽大肠杆菌O78感染导致的肉鸡生产性能下降,通过调节肠道菌群结构和机体氧化状态,来减轻大肠杆菌感染造成的生理失衡。提示饲粮添加200 mg/kg屎肠球菌可有效预防肉鸡大肠杆菌O78感染。
[Abstract]:The purpose of the experiment was to study the effects of dietary supplementation of Enterococcus faecium on the performance, intestinal microflora and blood antioxidant function of broilers infected with avian Escherichia coli O78. 288 1-day-old Roth308 hens were selected. Each group was randomly divided into 4 groups. Each group was fed with 0,100,200,300 mg/kg Enterococcus faecium in the basal diet. Each group was inoculated with 0,100,200,300 mL/ of Escherichia coli at the 11th day of age. The results showed that: compared with the control group, the diet supplemented with fecal intestines. The body weight of 10-day-old broilers was increased linearly, the body weight of 10-day-old broilers was increased linearly, the linear and quadratic linear increments were increased by 22 and 35 days, and the average daily gain of broilers at 10 ~ 10 ~ (10 ~ 22) and 10 ~ (35) days was increased by a linear way. The mortality rate of infected broilers was decreased by adding Enterococcus faecium to the diet. The number of Escherichia coli and Salmonella in cecum was decreased linearly 3 days after infection, the number of bifidobacterium was decreased by linear and quadratic linearity, and the number of Escherichia coli and Salmonella in Enterococcus faecium was decreased linearly at 7 days after infection. On the 7th day, the serum total antioxidant capacity of Enterococcus faecium group was linear (P 0.05) and quadratic linear decrease (P 0.05), malondialdehyde content was linear and quadratic linear decrease (P 0.05), glutathione peroxidase activity was quadratic linear decrease (P 0.05). It can be seen that diet supplemented with Enterococcus faecium can alleviate the decline in the performance of broilers caused by the infection of Escherichia coli O78 in poultry. The physiological imbalance caused by Escherichia coli infection was alleviated by regulating the intestinal flora structure and the oxidation state of the body, suggesting that the addition of 200 mg/kg Enterococcus faecium to diet could effectively prevent the infection of Escherichia coli O78 in broilers.
【作者单位】：中国农业大学动物医学院;中国农业大学动物科技学院;
【基金】：基金项目:现代农业产业技术体系北京市家禽创新团队(CARS-PSTP)
【分类号】：S858.31

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