普氏蹄蝠胃肠道细菌的分离与鉴定

发布时间：2018-03-13 14:46

本文选题：普氏蹄蝠　切入点：胃肠道细菌　出处：《动物医学进展》2017年07期 　论文类型：期刊论文

【摘要】：国内外关于蝙蝠消化道细菌的研究极少,为研究普氏蹄蝠(Hipposideros pratti)胃肠道细菌的种类及数量,对普氏蹄蝠胃肠道内的细菌进行分离,采用形态观察、生理生化试验、16S rRNA基因序列及序列同源性分析等方法,鉴定细菌的种类。结果表明,普氏蹄蝠胃肠道中存在的菌群隶属于肠杆菌属(Enterobacter)、克雷伯菌属(Klebsiella)、摩根菌属(Morganella)、哈夫尼菌属(Hafinia)、Gibbsibela、Lysinibacillus、乳球菌属(Lactococcus)、柠檬酸杆菌属(Citrobacter)、Raoultella、普罗威登斯菌属(Providencia)及肠球菌属(Enterococcus)共11个属。其中革兰阴性菌占据了绝对优势,普氏蹄蝠胃内可培养的细菌数量大约为3.8×10~8 CFU/mL~7.2×10~8 CFU/mL,肠道可培养的细菌数量约8.6×10~8 CFU/mL~1.3×10~(10)CFU/mL。普氏蹄蝠胃肠道中的细菌大多为人类致病菌或条件致病菌。其结果为普氏蹄蝠胃肠道细菌的深入研究奠定了基础,并为人与蝙蝠共患疾病的预防提供基础资料。
[Abstract]:There are few studies on the bacteria in the digestive tract of bats at home and abroad. In order to study the species and quantity of bacteria in the gastrointestinal tract of Hipposideros prattii, the bacteria in the gastrointestinal tract of Rhinolophus prattii were isolated and observed by morphological observation. In physiological and biochemical tests, the 16s rRNA gene sequence and sequence homology analysis were used to identify the species of bacteria. There are 11 genera in the gastrointestinal tract of Rhinolophus przewalskii, belonging to Enterobacterus, Klebsiellaa, Morganellaus, Hafiniaella, Lysinibacillus, Lactococcus, Citrobacillus, Providenciaand Enterococcus. Middle Gram-negative bacteria were dominant. The number of culturable bacteria in the stomach of Rhinolophus przewalskii was about 3. 8 脳 10 ~ (8) CFU/mL~7.2 脳 10 ~ (8) CFU / mL, and the number of bacteria in the intestine was about 8. 6 脳 10 ~ (8) CFU/mL~1.3 脳 10 ~ (10) CFU/mL~1.3 脳 10 ~ (10) CFU / m ~ (L). Most of the bacteria in the gastrointestinal tract of Rhinolophus przewalskii were human pathogenic bacteria or conditioned pathogens. The deep study of bacteria laid the foundation, It also provides basic data for the prevention of human and bat diseases.
【作者单位】：河南师范大学生命科学学院;
【基金】：国家自然科学基金项目(30970331,31172056) 河南省科技攻关项目(082102360004)
【分类号】：S852.61

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