鸭源鸡杆菌外膜蛋白A基因克隆及结构与功能分析

发布时间：2018-03-13 17:48

  本文选题：鸭源鸡杆菌　切入点：OmpA基因　出处：《河南农业科学》2017年08期 　论文类型：期刊论文

【摘要】：为筛选鸭源鸡杆菌(G.anatis)潜在的保护性抗原基因,参考Gen Bank中G.anatis UMN179的外膜蛋白A(OmpA)基因序列设计1对引物,对鸭源鸡杆菌PDS-RZ-1-SLG(RZ)株的OmpA基因进行克隆,并应用相关软件对其核苷酸及氨基酸序列进行生物信息学分析。结果显示,OmpA基因大小为1 083 bp,编码360个氨基酸;鸭源鸡杆菌RZ株的OmpA与UMN179、F149及12656/12的OmpA氨基酸相似性分别为93.2%、95.2%、92.7%;OmpA蛋白分子质量为38.4 ku,等电点为6.77,具有稳定性,N端有1个疏水性的α螺旋信号肽。
[Abstract]:In order to screen the potential protective antigen gene of G. anatisa, a pair of primers were designed to clone the OmpA gene of PDS-RZ-1-SLGG RZ strain from Gen Bank, referring to the gene sequence of G. anatis UMN179 outer membrane protein. The sequence of nucleotide and amino acid was analyzed by bioinformatics, and the result showed that the size of OmpA gene was 1 083 BP, encoding 360 amino acids. The similarity of OmpA amino acids between OmpA and UMN179 F149 and 12656/12 in RZ strain was 93.22.2 and 95.2%, respectively. The molecular weight of OmpA protein was 38.4 ku. the isoelectric point was 6.77. There was a hydrophobic 伪 helix signal peptide at the N terminal of RZ strain.
【作者单位】： 河南农业大学牧医工程学院;
【基金】：国家自然科学基金项目(3177130375) 河南省高校科技创新团队与支持计划资助项目(14IRTSHN015)
【分类号】：S852.61
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本文编号：1607469