江苏省禽源空肠弯曲杆菌的分离鉴定及耐药性研究

发布时间：2018-03-14 03:07

本文选题：江苏省　切入点：空肠弯曲杆菌　出处：《中国人兽共患病学报》2017年06期 　论文类型：期刊论文

【摘要】：目的了解江苏省禽源空肠弯曲杆菌的流行情况及耐药特征。方法从江苏徐州、淮安和扬州等7市采集禽肉产品和泄殖腔棉拭子样品共753份,分离细菌,PCR方法鉴定空肠弯曲杆菌后,根据WHO推荐的K-B法测定9大类27种抗生素的敏感性。结果 753份样品共分离出空肠弯曲杆菌207株,分离率为27.5%。药敏结果显示,所有菌株除了对美罗培南、庆大霉素、卡那霉素、氟苯尼考和磷霉素保持高度敏感性,对其它抗生素产生不同程度耐药,对磺胺类、喹诺酮类、β-内酰胺类和四环素类抗生素耐药率最高,其中对甲氧苄氨嘧啶、诺氟沙星、头孢曲松和四环素耐药率分别高达100%、84.02%、80.9%和79.4%,一株分离自徐州的菌株对92.6%的抗生素产生耐受性。测试菌株出现多重耐药现象,优势耐药谱为LIN/CTX/CRO/NOR/CIP/T/TE,菌株耐药类型在40%~60%数量最多,各市菌株耐药类型不一。结论江苏省禽源空肠弯曲杆菌耐药性比较普遍,多重耐药菌株较突出,应严格监管养殖场抗生素的使用及流通阶段食品源空肠弯曲杆菌的监测。
[Abstract]:Objective to understand the prevalence and drug resistance of avian Campylobacter jejuni in Jiangsu province. Methods from Jiangsu to Xuzhou, Huaian and Yangzhou 7 city collection of poultry products and cloacal swabs of 753 samples of isolated bacteria, PCR method for identification of Campylobacter jejuni, according to the sensitivity of determination of 9 major categories of 27 kinds of antibiotics K-B WHO recommended method the results of 753 samples were isolated from 207 strains of Campylobacter jejuni, the isolation rate of 27.5%. drug susceptibility test showed that all strains except for meropenem, gentamicin, kanamycin, florfenicol and fosfomycin to maintain a high degree of sensitivity, have different degrees of resistance to other antibiotics, quinolones, sulfonamides on beta. Lactam and tetracycline antibiotic resistance rate of trimethoprim, norfloxacin, ceftriaxone and tetracycline resistance rates were as high as 100%, 84.02%, 80.9% and 79.4%, a strain isolated from Xuzhou The 92.6% strains of antibiotic tolerance test strains. The emergence of multi drug resistance phenomenon, the dominant resistance spectra LIN/CTX/CRO/NOR/CIP/T/TE, drug-resistant strains of most types in 40%~60% number of drug-resistant strains in different cities of Jiangsu province. Conclusion avian Campylobacter jejuni widespread resistance, multidrug resistant strains is more prominent, should be strictly regulated farms and circulation stage the food source of Campylobacter antibiotic monitoring.

【作者单位】：中国农业科学院家禽研究所;
【基金】：十三五国家重点研发计划高危金属中毒病防控技术研究(2016YFD0501208) 扬州市社会发展(YZ2016058) 2017国家农产品质量安全风险评估重大专项(GJFP2017007) 扬州市家禽质量安全科技服务平台建设项目(yz2015162)联合资助~~
【分类号】：S852.61

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