四川兔源金黄色葡萄球菌毒力检测及分子分型

发布时间：2018-03-14 06:33

本文选题：金黄色葡萄球菌　切入点：家兔　出处：《华南农业大学学报》2017年04期 　论文类型：期刊论文

【摘要】：【目的】研究四川部分区域兔源金黄色葡萄球菌Staphylococcus aureus的基因型总体结构特征、遗传变异以及毒力因子的分布情况。【方法】从四川地区分离41株兔源金黄色葡萄球菌,鉴定fem B基因特异性,进行耐甲氧西林金黄色葡萄球菌(Methicillin-resistant S.aureus,MRSA)筛选,并通过PCR法检测13种常见的毒力基因,采用多位点序列分型(Multilocus sequence typing,MLST)和脉冲场凝胶电泳(Pulsed field gel electrophoresis,PFGE)确定基因型特征。【结果】41株金黄色葡萄球菌中共检测出MRSA 31株,检出率为75.61%;共检出9种毒力基因,其中nuc、hla、eta和clf A在所有菌株中均存在,而sea、sec、see、hlb和PVL的阳性检出率分别为9.7%、85.4%、80.5%、90.2%和7.3%。MLST分型结果显示,41株金黄色葡萄球菌只存在2种序列型(ST398、ST3320)和1个克隆群CC398,其中ST398为优势序列型,所占比例为97.6%。PFGE将41株金黄色葡萄球菌分为18个基因型,但不同区域间的基因型条带差异较小。【结论】四川调查区域兔源金黄色葡萄球菌毒力因子携带率较高,其对家兔的养殖业存在较大的安全威胁;分型分析说明四川部分区域金黄色葡萄球菌的主要流行菌株遗传变异程度小,菌株间亲缘关系较近。
[Abstract]:[objective] to study the genotype structure, genetic variation and virulence factor distribution of Staphylococcus aureus (Staphylococcus aureus) in Sichuan region. [methods] 41 strains of Staphylococcus aureus were isolated from Sichuan region. To identify the specificity of fem B gene, to screen methicillin-resistant S.aureus S. aureus, and to detect 13 common virulence genes by PCR. Multilocus sequence typing (MLST) and pulsed field gel electrophoresistics (PFGE) were used to identify the genotypes. [results] 31 strains of Staphylococcus aureus were detected, the detection rate was 75.61%, and 9 virulence genes were detected. The positive rates of nuclear hlaaneta and clf A were 90.2% and 7.30.50%, respectively, and the positive rates of hlb and PVL were 9.75.4and 80.5%, respectively. The results showed that there were only two sequence types ST398ST3320 and one clone CC398in the 41 strains of Staphylococcus aureus, in which ST398 was the dominant sequence type. The proportion was 97.6. PFGE divided 41 strains of Staphylococcus aureus into 18 genotypes, but there was little difference among different regions. [conclusion] the carrying rate of virulence factor of Staphylococcus aureus from rabbits in Sichuan Province is higher. The classification analysis showed that the genetic variation of the main prevalent strains of Staphylococcus aureus in some parts of Sichuan Province was small and the genetic relationship between strains was close.
【作者单位】：四川农业大学动物医学院;
【基金】：四川省科技支撑计划(2016NZ0002)
【分类号】：S852.611

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