蛋鸡沙门菌分离株生物被膜与耐药相关性研究

发布时间：2018-03-14 11:27

本文选题：沙门菌　切入点：生物被膜　出处：《中国家禽》2017年02期 　论文类型：期刊论文

【摘要】：为调查我国部分地区规模化蛋鸡场沙门菌生物被膜(BF)与耐药的相关性,2014~2015年从四川、云南、湖北、辽宁、天津、安徽六省规模化蛋鸡养殖场送检的病死鸡分离鉴定出40株沙门菌。玻片凝集法显示分离株包括6种血清型,其中以鼠伤寒沙门菌为主。结晶紫染色法检测生物被膜结果表明,15%(6/40)菌株形成强生物被膜;52.5%(21/40)菌株形成中强生物被膜;32.5%(13/40)菌株不形成生物被膜。K-B纸片法进行药敏试验的结果显示,产BF的27个沙门菌分离株对环丙沙星、诺氟沙星、恩诺沙星、复方新诺明、多西环素、四环素、链霉素、氨苄西林和青霉素9种药物均为多重耐药,耐药率100%,其中对多黏菌素B和头孢噻肟的敏感性显著降低。不产BF的13株沙门菌对以上受试抗生素药物均敏感,耐药率为0。结果表明蛋鸡沙门菌生物被膜与耐药存在相关性。
[Abstract]:In order to investigate the correlation between BFs of salmonella biofilm and drug resistance in some areas of China, from Sichuan, Yunnan, Hubei, Liaoning, Tianjin, Sichuan, Yunnan, Hubei, Liaoning, Tianjin, Sichuan, Yunnan, Hubei, Liaoning, Tianjin, Sichuan, Yunnan, Hubei, Liaoning, Tianjin, China. Forty strains of salmonella were isolated and identified from sick and dead chickens in six provinces of Anhui province. The slide agglutination method showed that the isolates included 6 serotypes. Among them, Salmonella typhimurium was the main one. The results of crystal violet staining for biofilm were as follows. The results of the drug sensitivity test showed that the biofilm was formed by 52.525 / 40) strain, and the strain 32.525 / 13 / 40) did not form biofilm. K-B disk method showed that the strain did not form biofilm. K-B disk method showed that the biofilm was not formed. The 27 strains producing BF were multidrug resistant to ciprofloxacin, norfloxacin, enrofloxacin, compound sulfamethoxine, doxycycline, tetracycline, streptomycin, ampicillin and penicillin. The sensitivity to polymyxin B and cefotaxime was significantly decreased. The 13 strains of Salmonella that did not produce BF were all sensitive to the antibiotics tested above, and the resistance rate was 0. The results showed that there was a correlation between the biofilm of Salmonella and the drug resistance of laying hens.
【作者单位】：四川大学生命科学学院动物疫病防控与食品安全四川省重点实验室生物资源与生态环境教育部重点实验室;
【基金】：国家重点研发计划项目(2016YFD0501608) 国家自然科学基金项目(31572547) 肉鸡现代产业链关键技术集成研究与产业化示范项目(2016NZ0003) 现代农业产业技术体系建设专项资金(CARS-41-K09)
【分类号】：S852.61

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