杜洛克猪HMGA1基因多态位点与生长、饲料利用性状的关联分析

发布时间：2018-03-14 18:36

本文选题：HMGA基因　切入点：杜洛克猪　出处：《中国畜牧兽医》2016年12期 　论文类型：期刊论文

【摘要】：试验对HMGA1基因的两个SNPs位点进行分析,研究了HMGA1基因核苷酸多态性与生长、饲料利用性状的关联性。利用大白猪和民猪重测序结果比较发现了HMGA1基因的两个SNPs位点(g.-543 TC和g.1356CT),利用Sequenom质谱测序平台对杜洛克猪g.-543TC和g.1356CT位点进行基因分型,并分析该位点多态性与生长、饲料利用性状的关联性。结果发现,杜洛克猪群体g.-543TC位点,CT与CC基因型相比,90日龄体重升高了2.15kg(P0.05),30kg体重日龄降低了3.21d(P0.05),断奶重升高,剩余采食量降低,100kg体重日龄减少,30~100kg平均日增重、40~90kg平均日采食量、40~90kg平均日增重均降低。杜洛克猪群体g.1356CT位点,CC与TT基因型相比,90日龄体重升高了0.37kg(P0.05);平均日采食量降低0.13kg/d(P0.05),100kg背膘厚降低0.43mm(P0.05),剩余采食量降低了70.42g(P0.05),30kg体重日龄降低了0.56d(P0.05);40~90kg平均日采食量降低了0.17kg(P0.05),断奶重升高,100kg体重日龄增大,30~100、40~90kg平均日增重均降低;CT与TT基因型结果与上结果类似。结果初步表明HMGA1基因两个SNPs位点的突变有利于杜洛克猪的生长、饲料利用效率的提高。
[Abstract]:Two SNPs loci of HMGA1 gene were analyzed and the nucleotide polymorphisms and growth of HMGA1 gene were studied. The correlation of feed utilization traits. Two SNPs loci of HMGA1 gene were found by comparing the results of heavy sequencing of large white pig and Min pig. The loci of g.-543TC and g.1356CT of Duroc pigs were genotyped by Sequenom mass spectrometry sequencing platform. The results showed that the body weight of Duroc pigs was increased by 2.15 kg / kg P0.05 / 30kg body weight and increased by 3.21 days / 30 kg / day, and the weaning weight was also increased compared with CC genotype (P < 0.05), and the correlation between the polymorphism and growth and feed utilization traits was also analyzed in Duroc pig population (g. -543TC), the results showed that compared with CC genotype, the weight of Duroc pigs increased at 90 days. The average daily gain of Duroc pigs decreased by 4090kg / day. Compared with TT genotype, CC increased 0.37 kg / day and decreased 0.37 kg / kg P0.05kg / day in Duroc pig population (g / 1356CT / kg / kg / kg / kg / kg / day / day / day / day / day / day / day / day / day / day group / day / day / day / day / day / day / day / day / day / day / day / day / day average daily intake decreased. The average daily intake decreased by 0.13 kg / d / d P 0.05 / 100 kg / day, by 0.43 mm / L / 100 kg / day, and by 70.42 g / g / kg / kg / kg / kg / day / day by 0.56 kg / kg / kg / kg / day / day / day / day / day / day respectively. The average daily intake decreased by 0.17 kg / d / 100 kg / day / day / day / day / day / day / day / day / day / day / day / day / day / day / day / day / day / day / day / day / day / day / day, respectively. The results of CT and TT genotypes were similar to those of the previous results. The results showed that the mutation of two SNPs loci of HMGA1 gene was beneficial to the growth of Duroc pigs. The improvement of feed utilization efficiency.
【作者单位】：中国农业科学院北京畜牧兽医研究所;天津农学院动物科学与动物医学学院;湖南农业大学动物科学技术学院;
【基金】：中国农业科学院科技创新工程(ASTIP-IAS02) 国家“十二五”科技支撑计划项目(2015BAD03B02-2) 国家现代农业产业技术体系(CARS-36)
【分类号】：S828

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