乳酸菌和纤维素酶对不同含水量紫花苜蓿青贮品质的影响

发布时间：2018-03-15 06:23

本文选题：苜蓿青贮　切入点：含水量　出处：《动物营养学报》2017年05期 　论文类型：期刊论文

【摘要】：本试验旨在探讨含水量和添加剂对紫花苜蓿青贮品质的影响,以期解决苜蓿青贮困难的问题,提高其青贮品质。采用双因素(含水量×添加剂)完全随机设计,含水量分别为70%和60%;添加剂分4个组,对照组不含添加剂,其余各组均含3个水平,分别为乳酸菌组(LA组,3、6、9 mg/kg)、纤维素酶组(CE组,25、50、100 mg/kg)以及乳酸菌和纤维素酶混合组(LA×CE组,3×25、6×50、9×100 mg/kg),每个组3个重复,共20个组。青贮90 d后,测定其粗蛋白质(CP)、粗纤维(CF)、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)、可溶性糖(WSC)含量及pH、氨态氮/总氮(AN/TN)。结果表明:与对照组相比,各LA组均显著提高了青贮料的WSC含量(P0.05),显著降低了青贮料pH(P0.05);且6 mg/kg LA组青贮料的CP和WSC含量高于3和9mg/kg LA组,pH低于3和9 mg/kg LA组。与对照组相比,各CE组均显著降低了青贮料的ADF含量和pH(P0.05),且50 mg/kg CE组青贮料的ADF含量和pH低于25和100 mg/kg CE组。各LA×CE组青贮料的CF、ADF含量和pH大部分低于各LA和CE组,WSC含量均高于各LA组和CE组,且6×50 mg/kg LA×CE组青贮料的CF、ADF、WSC含量和pH与各LA和CE组差异显著(P0.05)。除9 mg/kg LA组和100 mg/kg CE组外,相同添加剂下70%含水量青贮料的CP含量显著低于60%含水量(P0.05);相同添加剂下70%含水量青贮料的CF和ADF含量显著高于60%含水量(P0.05)。由此可见,乳酸菌和纤维素酶及两者复合添加均对苜蓿的青贮品质有明显的改善作用,并以添加6×50 mg/kg乳酸菌和纤维素酶效果最好,且60%含水量下的青贮效果更优。
[Abstract]:The purpose of this experiment was to investigate the effect of water content and additive on alfalfa silage quality, in order to solve the problem of alfalfa silage difficulty and improve the silage quality of alfalfa silage. The water content was 70% and 60, respectively. The additive was divided into 4 groups, the control group did not contain any additives, and the other groups contained 3 levels. Lactic acid bacteria group (LA group), lactic acid bacteria group (LA group), LA group (9 mg / kg), cellulase group (CE group), 25 50g / kg group (100 mg / kg) and lactic acid bacteria and cellulase mixed group (LA 脳 CE group, 3 脳 2510 脳 50 脳 10 mg / kg), 3 repeats in each group, and 20 groups were aged 90 days. The contents of crude protein (CP), crude fiber (CF), neutral detergent fiber (NDF), acid detergent fiber (ADF), soluble sugar (WSCC), pH, ammonia nitrogen / total nitrogen (TN) were determined. The results showed that: compared with the control group, All LA groups significantly increased the WSC content of the silage and decreased the pH value of P0. 05%, and the CP and WSC contents of the 6 mg/kg LA group were higher than those of the 3 and 9 mg / kg LA groups, and the pH value of the 6 mg/kg LA group was lower than that of the 3 and 9 mg / kg LA groups. The ADF content and pH value of the silage in the 50 mg/kg CE group were significantly lower than those in the LA 脳 CE group, and the ADF content and pH value of each LA 脳 CE group were significantly lower than those of the LA 脳 CE group and the pH value of each LA 脳 CE group were higher than those of the LA and CE groups, and the ADF content and pH value of the 50 mg/kg CE group were significantly lower than those of the LA 脳 CE group. The content and pH of CFO ADFN WSC in 6 脳 50 mg/kg LA 脳 CE group were significantly different from those of each LA and CE group (P 0.05), except 9 mg/kg LA group and 100 mg/kg CE group. The CP content of the silage with 70% water content under the same additive was significantly lower than that of the silage with 60% water content and the CF and ADF contents of the silage with 70% water content under the same additive were significantly higher than that of the silage with 60% water content. The silage quality of alfalfa was improved obviously by adding lactic acid bacteria, cellulase and their combination, and the silage effect was better when 6 脳 50 mg/kg lactic acid bacteria and cellulase were added, and the silage effect was better when the water content was 60%.
【作者单位】：西北农林科技大学动物科技学院;
【基金】：基金项目国家牧草产业技术体系(CARS-35-01A) 国家绒毛用羊产业技术体系(CARS-40-13) 国家燕麦产业技术体系(CARS-08-D1)
【分类号】：S816.53

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