两种缓解马应激中草药方剂的小鼠急性毒性试验

发布时间：2018-03-15 07:15

  本文选题：中草药方剂　切入点：小鼠　出处：《家畜生态学报》2017年08期 　论文类型：期刊论文

【摘要】：为研究两种缓解马应激中草药方剂的安全性,进行了中草药对小鼠的急性毒性试验。配制Ⅰ、Ⅱ号两种中草药方剂,各设高、中、低3个剂量试验组和1个空白对照组,每天灌服1次,连续灌服7d,每天观察小鼠行为等情况,7d后,采集血液和组织脏器,测定血常规并观察组织病理变化。结果显示:1)Ⅰ号方剂与对照组相比,小鼠WBC、GRAN%、RBC等血液指标显著降低(P0.05),高剂量组肝脏脏器系数极显著降低(P0.01),中剂量组肝脏脏器系数显著降低(P0.05),各剂量组肝脏病理变化呈现由强到弱趋势;2)Ⅱ号方剂与对照组相比,小鼠WBC、GRAN%等血液指标显著降低(P0.05),高、中剂量组肝脏的脏器系数均显著降低(P0.05),仅高剂量组肝脏有病理变化。结论:Ⅰ号方剂对肝脏有毒副作用,作用大小呈现剂量依赖性;Ⅱ号方剂高剂量对肝脏有毒副作用,中、低剂量无毒副作用。
[Abstract]:In order to study the safety of two Chinese herbal remedies for relieving horse stress, the acute toxicity test of Chinese herbal medicine to mice was carried out. After 7 days of observation of the behavior of mice, blood and tissue organs were collected, blood routine was measured and pathological changes were observed. The results showed that: 1) compared with the control group. The blood indexes such as RBC of mice WBC+ GRANX and RBC were significantly decreased, the liver organ coefficient of high dose group was significantly lower than that of control group, and the liver organ coefficient of middle dose group was significantly lower than that of control group. The pathological changes of liver of each dose group showed a tendency from strong to weak) compared with the control group, the liver organ coefficient of the middle dose group was significantly lower than that of the control group, and compared with the control group, the liver pathological changes of each dose group showed a tendency from strong to weak. The liver organ coefficient of middle dose group was significantly lower than that of high dose group, only the liver of high dose group had pathological changes. Conclusion: formula I has toxic side effect on liver, and the effect is dose-dependent. The high dose of prescription 鈪，

本文编号：1614979