新疆南疆部分规模奶牛场BVD流行病学调查及分析

发布时间：2018-03-16 00:01

本文选题：牛病毒性腹泻病　切入点：ELISA　出处：《塔里木大学》2017年硕士论文　论文类型：学位论文

【摘要】：牛病毒性腹泻病(Bovine viral diarrhea,BVD)是由牛病毒性腹泻病毒(Bovine viral diarrhea virus,BVDV)感染引起的一种接触性传染病,根据感染毒株的不同可引起牛表现多种临床症状,以粘膜溃疡、糜烂及腹泻为主要特征。近年来,BVD在新疆地区呈现蔓延趋势,严重影响了新疆规模养牛业的持续健康发展,因此掌握本地区BVD的流行现况对防控该病具有重要意义。1.2015年新疆南疆13个规模奶牛场124份大罐奶样进行BVDV抗体ELISA检测,其中3个奶牛场抗体阳性检出率为77.78%~82.35%,10个奶牛场抗体检出率达到100%。2.2015、2016年度新疆南疆14个规模奶牛场1555份血清样品进行BVDV抗体、抗原ELISA检测,2015年A~N奶牛场BVDV抗体阳性率84.48%以上,抗原阳性率为0.78%;2016年A~N奶牛场BVDV抗体阳性率91.38%以上,抗原阳性率为0.25%。3.RT-PCR检测共检出9份阳性样品。其中6株为BVDV Ic型,3株为BVDV I型未知亚型。表明新疆南疆规模化奶牛场存在BVDV Ic型和BVDV I型未知亚型。4.2016年9~10月3个规模奶牛场母牛、犊牛血清亲子样品各136份进行ELISA检测,犊牛均为抗原阴性,仅有1头母牛为抗原阳性并经RT-PCR检测,为BVDV I型未知亚型。本研究通过在新疆南疆部分规模奶牛场大罐奶样BVD检测、血清学流行病学调查、分子流行病学调查等,获得新疆南疆部分规模奶牛场BVD流行病学资料及流行株基因型,为规模奶牛场BVD免疫及控制策略提供参考依据。
[Abstract]:Bovine viral diarrhea (BVD) is a contact infectious disease caused by bovine viral diarrhea virus (BVDV) infection. It can cause many clinical symptoms and mucosal ulcers according to the different strains of bovine viral diarrhea. Erosion and diarrhea are the main characteristics. In recent years, BVD has been spreading in Xinjiang, which has seriously affected the sustainable and healthy development of large-scale cattle farming in Xinjiang. Therefore, it is important to know the prevalence of BVD in this area. 1. In 2015, 124 large pot milk samples from 13 large dairy farms in Southern Xinjiang were detected for BVDV antibody ELISA. The positive rate of antibody in 3 dairy farms was 77.78 and 82.35, and the positive rate of 10 dairy farms reached 100.2.2015. 1555 serum samples from 14 scale dairy farms in Nanjiang of Xinjiang were tested for BVDV antibody and antigen ELISA. In 2015, the positive rate of BVDV antibody in An dairy farm was more than 84.48%. In 2016, the positive rate of BVDV antibody in Agnon dairy farm was more than 91.38%. The positive rate of antigen was 0.25. 3. 9 positive samples were detected by RT-PCR, of which 6 were BVDV Ic type and 3 were unknown BVDV I subtypes, which indicated that there were unknown BVDV I and BVDV I subtypes in large-scale dairy farms in southern Xinjiang. 4. From 9 to October 2016, 3 dairy cows of large scale were identified. The serum samples were tested for ELISA, and only 1 cow was antigen-positive and tested by RT-PCR for unknown BVDV I subtype. In this study, BVD was detected in large pot milk samples of part scale dairy farms in southern Xinjiang. Serological epidemiological investigation and molecular epidemiological investigation were conducted to obtain BVD epidemiological data and genotypes of epidemic strains in some large-scale dairy farms in southern Xinjiang. The results provided a reference basis for BVD immunization and control strategies in large-scale dairy farms.
【学位授予单位】：塔里木大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.23

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