ESR、OPN和RBP4基因多态性对大白猪繁殖性状的影响

发布时间：2018-03-16 04:11

本文选题：ESR基因　切入点：OPN基因　出处：《中国畜牧兽医》2017年01期 　论文类型：期刊论文

【摘要】：为了探讨雌激素受体(estrogen receptor,ESR)、骨桥蛋白(osteopontin,OPN)和视黄醇结合蛋白4(retinol binding proteins 4,RBP4)的多态性对大白猪繁殖性状的影响,本研究采用PCR-RFLP技术检测试验猪群ESR、OPN和RBP4基因的多态性,并分析其对大白猪总产仔数、产活仔数、健仔数、窝重、初生重、死胎、木乃伊、弱仔数和畸形数等性状的影响。结果显示,在大白经产母猪中,ESR和OPN基因的优势基因为A等位基因,RBP4基因的优势基因为B等位基因;ESR基因AB与BB基因型在总产仔数、产活仔数性状上均高于AA基因型;OPN基因在总产仔数性状上AA基因型最高,在产活仔数、健仔数、窝重性状上均为BBAAAB;RBP4基因在总产仔数、产活仔数、健仔数、窝重性状上均为AABBAB;合并基因型分析发现ESR-RBP4最优的分子标记组合为ABAA;OPN-RBP4最优的分子标记组合为BBAA。因此,ESR、OPN和RBP4基因多态性与大白猪繁殖性状相关。
[Abstract]:In order to investigate the effect of polymorphism of estrogen receptor estrogen receptor (ER), osteopontin (OPN) and retinol binding proteins (RBP4) on reproductive traits of large white pigs, PCR-RFLP technique was used to detect the polymorphisms of ESR-OPN and RBP4 genes. The effects on total litter size, live litter size, healthy litter size, litter weight, birth weight, stillbirth, mummy, weak litter size and deformity number of large white pigs were analyzed. The dominant gene of ESR and OPN gene was the dominant gene of A allele and RBP4 gene because AB and BB genotypes of B allele ESR gene were in the total litter size. The gene of OPN was higher than that of AA genotype in total litter size traits. The gene of BBAAABABRBP4 gene was the highest in total litter size, healthy litter size, litter weight, and total litter size, and was the highest in total litter size, live litter size and healthy litter size. Combined genotypic analysis showed that the best molecular marker combination of ESR-RBP4 was ABAAOPN-RBP4, and the best molecular marker combination was ABBAA. Therefore, the polymorphism of ESR-OPN and RBP4 genes was associated with reproductive traits of large white pigs.
【作者单位】：石河子大学动物科技学院;新疆正大食品有限公司;
【基金】：国家国际科技合作项目(2014DFA31840) 国家自然科学基金(31460586) 教育部留学归国人员科研启动基金((2013)1092) 石河子大学动植物育种专项(gxjs2013-yz06)
【分类号】：S828

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