辽宁猪源大肠埃希菌的分离鉴定与药敏试验

发布时间：2018-03-16 22:38

本文选题：猪源　切入点：大肠埃希菌　出处：《动物医学进展》2017年02期 　论文类型：期刊论文

【摘要】：为了调查辽宁省不同地区猪源大肠杆菌病的流行和耐药情况,无菌采集临床疑似大肠杆菌病病死猪或患病猪病料65份,经细菌分离培养及生化试验,共分离到65株大肠埃希菌,分离率为100%。对分离到的菌株进行药物敏感试验及超广谱β内酰胺酶(ESBLs)筛选和确证试验,结果显示,辽宁地区分离株均表现出不同程度的耐药,耐药菌株占98.46%,其中对四环素、青霉素及氨苄西林的耐药率分别为93.33%、81.54%和80.00%;对甲氧苄啶、磺胺异恶唑、氯霉素、恩诺沙星耐药性较高,分别为74.29%、74.29%、69.23%和68.57%;对米诺霉素及多粘菌素B较为敏感,耐药率仅为10%和9.23%;分离株多药耐药率较高,达92.31%。ESBLs确证试验结果发现,辽宁省分离株中29.23%的菌株能产生ESBLs。说明辽宁地区猪大肠埃希菌流行情况较为广泛,耐药情况较为严重。
[Abstract]:In order to investigate the prevalence and drug resistance of porcine Escherichia coli in different areas of Liaoning Province, 65 strains of Escherichia coli strains were isolated from clinically suspected dead pigs or sick pigs by bacterial isolation, culture and biochemical tests. The isolation rate was 100. Drug sensitivity test and extended spectrum 尾 -lactamase (ESBLs) screening and confirmatory test were carried out. The results showed that all the isolates in Liaoning area showed different degrees of drug resistance, and 98.46% of the isolates were resistant to tetracycline. The drug resistance rates of penicillin and ampicillin were 93.331.54% and 80.00.The drug resistance to trimethoprim, sulfamethoxazole, chloramphenicol and enrofloxacin were 74.290.29% and 68.57%, respectively. The rates of drug resistance were only 10% and 9.23.The multidrug resistance rate of the isolates reached 92.31%. The results showed that 29.23% strains in Liaoning Province could produce ESBLs. the results showed that the prevalence of Escherichia coli in Liaoning was more widespread and the drug resistance was serious.
【作者单位】：辽宁省动物疫病预防控制中心/辽宁省动物医学研究院;辽宁省重大动物疫病应急中心;
【基金】：辽宁省自然科学基金项目(201402573) 辽宁省农业攻关及产业化项目(2015202013) 2014年中央财政农业科技推广示范项目(2130106)
【分类号】：S858.28

【相似文献】