免疫磁珠富集法对牛源大肠杆菌O157∶H7分离鉴定的影响

发布时间：2018-03-17 14:30

本文选题：免疫磁珠　切入点：大肠杆菌O∶H　出处：《中国畜牧兽医》2017年04期 　论文类型：期刊论文

【摘要】：为证明免疫磁珠吸附技术对牛源大肠杆菌O157∶H7分离效率的影响,从新疆五家渠市、伊宁县、昌吉市3个牛场的采集18份粪样、162份肛拭子、10份饲料样、17份水样和36份胴体表面棉拭子样本,经EC肉汤增菌后,分别采用免疫磁珠富集后和直接进行SMAC和MUG试验进行选择性培养,然后对菌体rfbE基因和鞭毛fliC基因进行PCR检测,最后对疑似大肠杆菌O157∶H7菌用生化试验进行符合性检测。结果2种方法分别从243份样品中分离到8株和4株大肠杆菌O157∶H7,统计学分析该差异不显著。本试验结果表明,在实践中免疫磁珠吸附技术和普通方法相比虽然在统计学上差异不显著,但确实能够增加大肠杆菌O157∶H7的分离数量,这与以前的相关研究结果基本一致。
[Abstract]:In order to prove the effect of immunomagnetic bead adsorption technology on the separation efficiency of bovine Escherichia coli O157: H7, this study was conducted from Wujiaqu City, Xinjiang, Yining County, Xinjiang. In 3 cattle farms of Changji, 18 feces and 162 anal swabs were collected. 17 samples of water and 36 samples of cotton swab on carcass surface were collected. After enrichment with immunomagnetic beads and direct SMAC and MUG tests, the cell rfbE gene and flagellum fliC gene were detected by PCR. Finally, the conformance test was carried out for suspected Escherichia coli O157: H7. Results 8 strains of Escherichia coli O157: H7 and 4 strains of Escherichia coli O157: H7 were isolated from 243 samples by two methods. In practice, the immunomagnetic bead adsorption technique is not statistically significant, but it does increase the isolation of E. coli O157: H7, which is consistent with previous studies.
【作者单位】：新疆农业大学动物医学学院;
【基金】：国家自然科学基金项目(31560485)
【分类号】：S852.61

【相似文献】