9株Ⅰ群禽腺病毒的分离鉴定及hexon基因的遗传变异分析

发布时间：2018-03-17 16:47

  本文选题：肉鸡　切入点：禽腺病毒　出处：《中国兽医杂志》2017年01期 　论文类型：期刊论文

【摘要】：为了解山东省肉鸡场禽腺病毒的感染流行特点,为其科学防治提供依据,通过临床症状观察、病毒分离鉴定、鸡胚致病性试验、血凝试验、PCR检测及克隆测序等方法进行病原检测分析。结果表明,发病肉鸡主要表现为精神沉郁、采食减少等临床症状。鸡胚致病性试验可见死亡胚体呈全身出血、体型小、肝肿大现象。血凝试验显示,分离株不具凝集鸡、鸭、大鼠红细胞的特性。对hexon基因进行克隆测序分析显示,LY9株与Ⅰ群禽腺病毒血清8型亲缘性近,与参考株(58株)的核苷酸同源性为98.8%,氨基酸同源性为98.6%。其余8株分离株间的核苷酸同源性为91.3%~99.8%,氨基酸同源性为97.8%~100%;与Ⅰ群禽腺病毒血清4型参考株(HB1510株)属同一进化分支,核苷酸同源性为93.1%~100%,氨基酸同源性为98.9%~100%。结论:从山东省4家肉鸡场分离到的9株病毒均为Ⅰ群禽腺病毒。
[Abstract]:In order to understand the epidemic characteristics of avian adenovirus infection in poultry farms in Shandong Province and provide scientific basis for its prevention and treatment, clinical symptom observation, virus isolation and identification, and chicken embryo pathogenicity test were carried out. The results showed that the main clinical symptoms of broiler disease were depression of mind and decrease of feed intake. The pathogenicity test of chicken embryo showed that the dead embryo body was bleeding all over the body and its size was small. Hepatomegaly. Hemagglutination test showed that the isolated strain did not possess the characteristics of agglutinated chicken, duck and rat red blood cells. Cloning and sequencing of hexon gene showed that LY9 strain was closely related to serotype 8 of group 鈪，

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