贵妃鸡与麒麟鸡微卫星遗传多样性研究

发布时间：2018-03-17 20:25

本文选题：微卫星　切入点：多态性　出处：《中国畜牧杂志》2017年02期 　论文类型：期刊论文

【摘要】：以岭南黄鸡为参照,利用微卫星位点检测贵妃鸡和麒麟鸡的遗传多样性,评估群体遗传资源现状。结果表明:26个微卫星位点在30个贵妃鸡、40个麒麟鸡和30个岭南黄鸡个体中共检测到197个等位基因,每个位点的等位基因范围为3个(MCW0037、MCW0165、MCW0098)~19个(LEI0234),平均7.58;总体平均期望杂合度为0.6816,平均观察杂合度0.5625,平均多态信息含量0.6327;岭南黄鸡的遗传多样性最高,麒麟鸡次之,贵妃鸡最低;3个群体均偏离了哈迪-温伯格平衡;贵妃鸡和麒麟鸡的群体内近交系数高于岭南黄鸡,表明前者的近亲交配事件较频繁;麒麟鸡群体内的遗传分化系数(均值0.2433)和岭南黄鸡(0.2281)大于贵妃鸡(0.0359),表明后者的有效群体较小。在后续的品种选育工作中应当加强亲本亲缘关系的评估,提高选育群体的有效数量,尽量减少近亲交配。
[Abstract]:The genetic diversity of Chicken Wings and Legs with Brown Sauce and Steamed Sliced Chicken with Mushrooms and Vegetables was detected by microsatellite locus using Lingnan Yellow Chicken as reference. The results showed that a total of 197 alleles were detected in 30 Chicken Wings and Legs with Brown Sauce, 40 Steamed Sliced Chicken with Mushrooms and Vegetables and 30 Lingnan Yellow chickens by 26 microsatellite loci. The allelic range of each locus was 3 MCW0037 / MCW0165 / MCW0098 ~ 19 (mean 7.58), the average expected heterozygosity was 0.6816, the average observed heterozygosity was 0.5625 and the average polymorphic information content was 0.6327.The genetic diversity of Lingnan Yellow Chicken was the highest, followed by Steamed Sliced Chicken with Mushrooms and Vegetables. Chicken Wings and Legs with Brown Sauce was the lowest, all the three populations deviated from the Hardi-Weinberg equilibrium, the inbreeding number of the two populations was higher than that of the Lingnan Yellow Chicken, which indicated that the inbreeding events of the former were more frequent than those of the Lingnan Yellow Chicken. The coefficient of genetic differentiation in Steamed Sliced Chicken with Mushrooms and Vegetables population (mean 0.2433) and Lingnan Yellow Chicken (0.2281) were larger than that of Chicken Wings and Legs with Brown Sauce 0.0359, which indicated that the effective population of the latter was smaller, and the evaluation of parental relationship should be strengthened in the subsequent breeding work. Increase the effective number of breeding population and reduce inbreeding as far as possible.
【作者单位】：嘉应学院生命科学学院;广东海洋大学农学院;
【基金】：种广东省自然科学基金项目(2014A030307018) 嘉应学院“创新强校工程”项目(CQX019);嘉应学院重点科技计划项目(2015KJM03) 广东省科技计划项目(2015A020208020、2016A030303068) 国家科技基础条件平台-特种动物子平台建设(201520)
【分类号】：S831.2

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