输血疗法在犬细小病毒病中的临床观察

发布时间：2018-03-18 03:34

本文选题：犬细小病毒　切入点：全血输入疗法　出处：《南京农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：随着时代的发展,宠物俨然成为我们家庭中重要的一员,宠物的健康关系到家庭的和谐。而犬的细小病毒感染目前对于幼犬危害比较大,尤其是胃肠出血性的犬细小病毒更是因为其发病过程中大量的失血及离子的丢失紊乱夺去了许多可爱鲜活的小生命。九十年代早期,国内有一些学者和科学试验机构将输血疗法应用于宠物临床,并取得了一定的成功,论文对犬血液在临床医疗上的筛选配型及采集储存作了详细的阐述,并用实例证明了输血疗法在提高犬小病毒的临床治愈率上的作用是很明显的。研究内容分为二个部分:1.犬血液在临床医疗上的筛选配型及采集储存在使用输血疗法救治病犬之前,需要对供血犬的品种和血型进行选择,要选择做过免疫的犬作为供血犬,然后进行血涂片、病毒、血液生化指标、体内及体表寄生虫方面的检测,检测完毕后进行犬血液交叉配血实验。分别从供体和受体采血1ml,并用EDTA抗凝,在离心机内离心1min,然后把受体和供体的红细胞分别配成2%红细胞混悬液,用生理盐水进行清洗,最后分别将供血犬的红细胞和受血者的血清,受血者的红细胞和供血者的血清混合,观察是否有凝聚反应。犬全血的储存主要指的是红细胞的保存,犬全血的采集一般是现采现输,通过在注射器里加入抗凝剂,在静脉采血后加入生理盐水或者糖盐水后100ml～200ml摇匀。2.全血输入疗法在犬细小病毒病治疗中的实际运用国内犬细小病毒感染发病率呈逐年递增趋势,且在防疫、诊断、治疗等方面存在很多问题,通过对犬细小病毒感染病犬的调查发现,全血小病毒的感染与犬龄、季节、品种和犬只是否免疫有关,犬只患犬细小病毒病表现的临床症状为:呕吐、腹泻、血便、精神状态差、食欲低下等。可采用犬细小病毒胶体金快速诊断试纸针对犬的粪便、生理指标和血气进行检测和诊断,确诊后通常采用注射细小病毒单克隆抗体或含细小病毒的高免血清治疗。本文还对全血输入法治疗犬细小病毒病犬的实例进行分析,实例中列举了不同品种、不同年龄、不同临床症状的犬只,分析发现,犬细小病毒的全血输入疗法在治疗犬出血性肠炎犬细小病毒病和多种病因结合的复杂性犬细小病毒病上具有很好的效果。
[Abstract]:With the development of the times, pets have become an important member of our family. The health of pets is related to the harmony of the family. However, the dog's parvovirus infection is now more harmful to puppies. In particular, gastrointestinal hemorrhagic canine parvovirus is the result of massive blood loss and ion loss disorders that have taken away many lovely, fresh, small lives in the early days of 90s. Some domestic scholars and scientific experimental organizations have applied transfusion therapy to pet clinic, and have achieved certain success. This paper expounds in detail the selection, matching, collection and storage of canine blood in clinical medicine. The effect of transfusion therapy on the clinical cure rate of canine small virus is proved to be very obvious. The research is divided into two parts: 1.The screening matching of canine blood in clinical medicine and the collection and storage in the use of blood transfusion therapy. Before the treatment of the sick dog, We need to select the breed and blood type of the blood supply dog, select the dog that has been immunized as the blood supplying dog, and then test the blood smear, virus, blood biochemical index, body surface parasite. The blood was collected from donor and recipient at 1ml, and then centrifuged in centrifuge for 1 min with EDTA. Then, the recipient and donor erythrocytes were mixed into 2% erythrocyte suspension respectively and washed with normal saline. Finally, the red blood cells of the dogs and the sera of the recipients, the red blood cells of the recipients and the sera of the donors were mixed to observe whether there was a condensation reaction. The storage of the whole blood of the dogs mainly refers to the preservation of the red blood cells. The collection of whole blood in dogs is generally collected and delivered, by adding an anticoagulant to the syringe. Application of whole blood transfusion therapy in the treatment of canine parvovirus infection the incidence of dog parvovirus infection is increasing year by year. There are many problems in treatment. Through the investigation of canine parvovirus infected dogs, it is found that the infection of whole blood virus is related to dog age, season, breed and whether the dog is immunized. The clinical symptoms of canine parvovirus disease are vomiting, diarrhea, blood stool, poor mental state, low appetite and so on. Physiological indexes and blood gas were detected and diagnosed. After diagnosis, monoclonal antibodies to parvovirus or high immune serum containing parvovirus were usually used to treat canine parvovirus disease, and the examples of whole blood transfusion in the treatment of canine parvovirus disease dogs were also analyzed in this paper. Examples of different breeds, different ages, different clinical symptoms of dogs, analysis found, The whole blood transfusion therapy of canine parvovirus has a good effect on the treatment of hemorrhagic enteritis canine parvovirus disease and complicated canine parvovirus disease with multiple etiological factors.
【学位授予单位】：南京农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.292

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