福建NADC30-like PRRSV FJLY01株的全基因组分子特征分析

发布时间：2018-03-18 09:14

本文选题：PRRSV　切入点：全基因组　出处：《西北农林科技大学学报(自然科学版)》2017年03期 　论文类型：期刊论文

【摘要】：【目的】了解福建省NADC30-like PRRSV FJLY01株的分子生物学特征和遗传演化规律,为PRRSV的防控提供参考。【方法】对采自福建省规模化猪场的疑似病料进行PRRSV分离鉴定,以NADC30PRRSV毒株基因序列为参考设计并合成9对引物,分别对分离株序列进行分段PCR扩增,经克隆测序后进行序列拼接,采用MEGA 6.0软件进行核苷酸及氨基酸同源性比对,并绘制其与其他35株毒株的系统遗传进化树。【结果】FJLY01株基因组全长为15 016bp(不包含polyA),包含10个开放阅读框,5′-UTR长190bp,3′-UTR长148bp,Nsp2基因缺失393bp。Nsp2蛋白存在类似PRRSV MN184和NADC30株的131个氨基酸的缺失。核苷酸比对结果表明,FJLY01株与VR-2332、MLV RespPRRS/Repro、CH-1a和BJ-4株核苷酸的同源性为85.2%~86.3%,与JXA1、HuN4等HP-PRRSV株核苷酸的同源性为83.7%~84.1%,与MN184A、MN184B株核苷酸的同源性为87.9%~88.2%,与NADC30株核苷酸的同源性最高,为97.1%。系统进化树分析表明,该毒株属于NADC30-like毒株,与JXA1、VR2332和CH-1a的亲缘关系较远。【结论】福建省存在NADC30-like毒株,应该加强PRRSV的监测。
[Abstract]:[Objective] to understand the molecular biology and genetic characteristics of Fujian province NADC30-like PRRSV FJLY01 strain evolution, to provide reference for PRRSV prevention and control. [Methods] collected from large-scale pig farms in Fujian province suspected disease material for isolation and identification of PRRSV gene sequence of NADC30PRRSV strain, with reference to the design and synthesis of 9 pairs of primers, respectively for isolates the sequence of segmented PCR amplification, sequencing of sequence of nucleotide and amino acid homology with MEGA 6 software, and draw it with the other 35 isolates of the phylogenetic tree. [results] the full-length genome of FJLY01 strain was 15 016bp (excluding polyA), contains 10 open reading frame 5 '-UTR -UTR 3' long 190bp, long 148bp, deletion of Nsp2 gene deletion of 393bp.Nsp2 protein has 131 amino acids like PRRSV MN184 and NADC30 strains. The nucleotide alignment results showed that FJLY01 strain and VR-23 32, MLV, RespPRRS/Repro, CH-1a and BJ-4 the nucleotide homology was 85.2%~86.3%, and JXA1, HuN4 and HP-PRRSV the nucleotide homology was 83.7%~84.1%, MN184A and MN184B, the nucleotide homology was 87.9%~88.2%, and NADC30 the nucleotide homology, phylogenetic tree analysis showed that 97.1%. strain belonged to NADC30-like strains, the and, JXA1, VR2332 and CH-1a of the relationship is far. [Conclusion] in Fujian province NADC30-like strains, should strengthen the monitoring of PRRSV.

【作者单位】：龙岩学院生命科学学院福建省家畜传染病防治与生物技术重点实验室福建省生猪疫病防控工程技术研究中心;
【基金】：福建省科技厅重大专项专题项目(2014NZ0002) 福建省省属高校科研课题(JK2015049) 福建省自然科学基金项目(2016J01168) 福建省科技平台项目(2014N2011)
【分类号】：S852.65

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