奶牛乳腺上皮细胞CPT2基因与脂质代谢关系的研究

发布时间：2018-03-19 02:18

本文选题：CPT2基因　切入点：奶牛乳腺上皮细胞　出处：《吉林农业大学》2017年硕士论文　论文类型：学位论文

【摘要】：牛奶是一种含有丰富营养物质的饮品,也是人体内钙的最佳来源。随着我国奶牛业的迅猛发展,牛奶渐渐受到人们的青睐,低脂健康牛奶逐渐成为人们的首选。肉碱棕榈酰基转移酶2(Carnitine Palmitoyltransferase II,CPT2)基因是CPT家族中的一个亚型,CPT2蛋白位于线粒体内膜,是线粒体β-氧化的限速酶。CPT2基因来源于课题组前期针对高乳脂高蛋白荷斯坦奶牛与低乳脂低蛋白荷斯坦奶牛转录组学分析的基础上,筛选出的差异基因。本研究构建了CPT2基因sh RNA干扰载体和过表达载体,通过瞬时转染法转入到奶牛的乳腺上皮细胞(Mammary Epithelial Cells,MEC)中,同时进行了m RNA水平和蛋白水平的验证,并测定了细胞内甘油三酯的含量和脂肪酸含量的变化。结果表明,干扰CPT2基因后,奶牛乳腺上皮细胞内甘油三酯含量与对照组相比显著性升高(p=0.01930.05);CPT2基因表达水平与长链脂肪酸含量正相关,干扰组与对照组相比脂肪酸含量显著降低(p=0.02470.05),过表达组与对照组相比脂肪酸含量显著性升高(p=0.04470.05)。通过本研究,我们明确了CPT2基因的对脂质代谢的影响,为日后构建特殊群体中国荷斯坦奶牛奠定了基础。
[Abstract]:Milk is a rich nutrient drinks, is the best source of calcium in the body. Along with the rapid development of China's dairy industry, milk gradually favored, low-fat milk has gradually become the first choice for people. Carnitine palmitoyl transferase (Carnitine Palmitoyltransferase 2 II, CPT2) gene is a subtype of the CPT family the CPT2 protein located in the inner mitochondrial membrane, is the rate limiting enzyme of.CPT2 gene from mitochondrial beta oxidation in ourlaboratory against high fat high protein and low fat low Holstein Holstein group protein transcription analysis, differential gene screening. This study constructed RNA interference vector of CPT2 gene and sh expression vector into cow mammary epithelial cells by transient transfection method (Mammary Epithelial Cells, MEC), and verify the m RNA and protein level, and test The change of intracellular triglyceride content and fatty acid content. The results show that the interference of CPT2 gene, triglyceride content in bovine mammary epithelial cells compared with the control group increased significantly (p=0.01930.05); the expression level of CPT2 gene with long chain fatty acid content is related to the interference group compared with the control group, the fatty acid content decreased significantly (p=0.02470.05), over expression group compared with the control group, the fatty acid content increased significantly (p=0.04470.05). Through this study, we identified the CPT2 gene effect on lipid metabolism, lays the foundation for constructing the special group of Chinese Holstein day.

【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S823

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