3～6月龄伊犁马肠道微生物群落多样性的研究

发布时间：2018-03-19 12:18

本文选题：伊犁马　切入点：Illumina　出处：《动物营养学报》2017年05期 　论文类型：期刊论文

【摘要】：本试验旨在研究3~6月龄伊犁马肠道微生物群落的多样性,揭示断奶前马驹肠道菌群演替的规律,从微生物学角度为此阶段马驹生长发育及肠道健康研究提供理论依据。试验选取平均体重为(89.75±8.81)kg、出生日期相同的3月龄伊犁马5匹,进行为期90 d的饲养试验。分别在试验的第0天(即试验开始的前1天)、第30天、第60天、第90天采集马驹粪便样品,从每份样品中提取微生物基因组总DNA,采用Illumina HiSeq测序技术检测样品微生物群落多样性。结果显示:1)对5匹马驹的20份粪样进行测序,共获得有效序列数157 665条,获得平均操作分类单元(OTUs)1 117个。2)马驹粪便中微生物群落α多样性指数(ACE、Chao1、Shannon和Simpson指数)随着马驹月龄的增加呈波动性变化,但各时间点之间均无显著性变化(P0.05)。3)在门水平上,马驹粪便中10大优势菌分别为厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)、变形菌门(Proteobacteria)、疣微菌门(Verrucomicrobia)、螺旋体菌门(Spirochaetes)、梭杆菌门(Fusobacteria)、无壁菌门(Tenericutes)、放线菌门(Actinobacteria)、TM7、广古菌门(Euryarchaeota),其中以厚壁菌门、拟杆菌门、变形菌门及疣微菌门的丰度较高;在科水平上,马驹粪便中10大优势菌分别为芽孢杆菌科(Bacillaceae)、莫拉氏菌科(Moraxellaceae)、动球菌科(Planococcaceae)、肉杆菌科(Carnobacteriaceae)、BS11、RFP12、乳杆菌科(Lactobacillaceae)、瘤胃球菌科(Ruminococcaceae)、毛螺旋菌科(Lachnospiraceae)、紫单胞菌科(Porphyromonadaceae);在属水平上,马驹粪便中10大优势菌分别为不动杆菌属(Acinetobacter)、德库菌属(Desemzia)、乳杆菌属(Lactobacillus)、Ureibacillus、Paludibacter、芽孢杆菌属(Bacillus)、埃希式杆菌属(Escherichia)、肉食杆菌属(Carnobacterium)、密螺旋体属(Treponema)、艰难杆菌属(Mogibacterium)。由此得出,采用Illumina HiSeq测序技术能够准确地对3~6月龄伊犁马肠道微生物群落进行分类研究;厚壁菌门、拟杆菌门、变形菌门、疣微菌门是3~6月龄伊犁马肠道内的优势菌门。
[Abstract]:The aim of this study was to study the diversity of intestinal microflora in the yili horse at the age of 3 and 6 months, and to reveal the succession of intestinal microflora before weaning. From the point of view of microbiology, the theoretical basis was provided for the study of growth, development and intestinal health of the foal at this stage. The average body weight was 89.75 卤8.81 kg, and 5 horses of the same birth date were 3 months old Yili horses. A 90-day feeding trial was conducted. Samples of foal dung were collected on day 0 (1 day before the trial, 30 days, 60 days, 90 days), respectively. The total genomic DNA of microorganism was extracted from each sample, and the diversity of microbial community was detected by Illumina HiSeq sequencing technique. The results showed that 20 feces from 5 ponies were sequenced, and the effective sequence number was 157,665. The average operating classification unit (OTUs1 117. 2) the 伪 -diversity index of microbial community in the feces of pony (ACE-Chao1 Shannon and Simpson index) fluctuated with the increase of foal age, but there was no significant change at each time point (P 0.05N. 3) at the gate level. The top 10 dominant bacteria in the feces of pony are Firmicuteschus, Bacteroidetesa, Proteobacteriae, Verrucomicrobiae, Spirochaetesa, Fusobacteria, Tenericuteschus, Actinobacteriae TM7, Euryarchaetataa. The abundance of the phylum Proteus and Microphylum verruca is higher; at the family level, The top 10 dominant bacteria in the feces of pony are Bacillaceaeae, Moraxellaceaeae, Planoacecoccae, Carnobacter aceaeus BS11RFP12, Lactobacillus aceaeae, Ruminococcaceaeae, Lachnospiraceaeae, Porphyromonadaceaeae; at the generic level, The dominant bacteria in the feces of pony are Acinetobacter Acinetobacter, Desemziaus, Lactobacillus Ureibacillus Paludibacterium, Bacillus, Escherichia coli, Carnobacterium, Treponemia, Mogibacterium iumum. Using Illumina HiSeq sequencing technique, the intestinal microflora of Yili horse at the age of 3 and 6 months could be accurately classified and studied, and the phylum platyphylum, Bacteroides phylum, Proteus phylum and Microphylum verrucum were the dominant microphylum in the intestinal tract of Yili horse at the age of 3 or 6 months.
【作者单位】：新疆农业大学新疆肉乳用草食动物营养实验室;
【基金】：“十二五”农村领域国家科技计划课题(2012BAD45B01)
【分类号】：S821

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