白藜芦醇对雄性生殖系统的抗氧化损伤研究

发布时间：2018-03-20 02:12

本文选题：白藜芦醇　切入点：猪睾丸间质细胞　出处：《湖南农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：白藜芦醇作为一种天然产物的提取物,研究发现它不但在抗肿瘤、抗心血管疾病、抗炎、抗菌抗病毒、抗衰老方面有影响,而且在生殖系统中也起着很重要的作用。为探究其抗氧化损伤作用,本论文通过体外细胞实验,并结合体内动物实验研究了白藜芦醇的抗氧化作用。体外细胞实验：通过建立猪原代睾丸间质细胞体系,用诱导剂过氧化氢建立了猪原代睾丸间质细胞的氧化损伤模型,一定剂量的(150 μM或以上)过氧化氢处理间质细胞能影响其形态学变化,抑制细胞的增殖,细胞发生凋亡的比例明显增加,相应的抗氧化成分超氧化物歧化酶SOD显著降低,而且细胞分泌睾酮能力显著下降；低剂量的白藜芦醇处理睾丸间质细胞可以促进细胞的增殖,浓度为0.2 gM的白藜芦醇对细胞作用效果较好；另外,用白藜芦醇可以在一定程度上修复由过氧化氢所引起的细胞氧化损伤,表现为：减少过氧化氢对细胞的抑制；提高氧化损伤细胞中抗氧化物质SOD的产生；同时提高氧化损伤细胞分泌睾酮的能力并减少细胞的凋亡。体外白藜芦醇的抗氧化损伤作用可为后续的体内动物实验提供基础。体内动物实验：选用未成年雄性小鼠为实验动物,用环磷酰胺建立小鼠生精障碍模型,生殖毒性作用比较明显,现为小鼠的睾丸指数和附睾指数显著下降；总精子数下降；精子畸形率升高；睾丸出现较明显的病理形态学变化；睾酮分泌下降；还影响小鼠的生长；当用白藜芦醇处理环磷酰胺造模的小鼠时,能在一定程度上减少其生殖毒性,表现为：体重增加、睾丸指数、附睾指数和精子总数显著增加,相应的精子畸形率减少、睾丸病理形态学变化减轻以及血清中睾酮含量增加,但血清中抗氧化成分SOD和谷胱甘肽酶GSH-Px没有显著变化；另外,单独用40 mg/kg · d剂量的白藜芦醇处理未成年雄性小鼠对其生殖不造成影响。以上结果表明：白藜芦醇对氧化损伤所致的生殖毒性有一定的治疗作用,但其具体作用机制还需进一步研究。相关结果白藜芦醇作为一种体内外抗氧化药物或作为一种饲料添加剂在畜牧生产中的应用提供参考。
[Abstract]:Resveratrol as a natural product of extract, research found that it not only in anti-tumor, anti-cardiovascular disease, anti-inflammatory, anti-viral, anti-aging effects, It also plays an important role in the reproductive system. The antioxidation effect of resveratrol was studied in vivo. In vitro cell experiment: the model of oxidative damage of primary testicular interstitial cells of pig was established by using hydrogen peroxide as an inducer. The interstitial cells treated with hydrogen peroxide at a certain dose of 150 渭 M or more could affect the morphological changes, inhibit the proliferation of the cells, increase the proportion of cell apoptosis, and decrease the SOD of superoxide dismutase (SOD). In addition, low dose resveratrol could promote the proliferation of testicular interstitial cells, and the concentration of 0.2 g / g resveratrol had a better effect on the cells. Resveratrol can repair the oxidative damage induced by hydrogen peroxide to some extent, such as reducing the inhibition of hydrogen peroxide on cells, increasing the production of antioxidant SOD in oxidative damage cells. The antioxidative effects of resveratrol in vitro may provide the basis for further in vivo animal experiments. In vivo animal experiments: juvenile males are selected. Mice are experimental animals, Cyclophosphamide was used to establish mouse model of spermatogenesis. The reproductive toxicity was obvious. The testicular index and epididymal index of mice were significantly decreased, the total sperm number was decreased, the sperm deformity rate was increased, the testis had obvious pathomorphological changes. The decrease in testosterone secretion also affected the growth of mice, and when resveratrol was treated with cyclophosphamide, the reproductive toxicity of mice was reduced to a certain extent, as follows: weight gain, testicular index, The epididymal index and total sperm count increased significantly, the corresponding sperm deformity rate decreased, the testicular pathomorphology decreased and the testosterone content in serum increased, but the antioxidant components SOD and glutathione GSH-Px did not change significantly. 40 mg/kg 路d of resveratrol alone had no effect on the reproduction of juvenile male mice. The results showed that resveratrol had a certain therapeutic effect on the reproductive toxicity induced by oxidative injury. Results resveratrol as an antioxidation drug in vivo and in vitro or as a feed additive in animal production provides reference for the application of resveratrol in animal husbandry.
【学位授予单位】：湖南农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S859.7

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