6株鸡柔嫩艾美耳球虫Cytb基因突变及其抗癸氧喹酯药物的差异分析

发布时间：2018-03-20 05:06

本文选题：柔嫩艾美耳球虫　切入点：Cytb　出处：《南京农业大学学报》2017年03期 　论文类型：期刊论文

【摘要】：[目的]研究细胞色素b基因(Cytb)突变与柔嫩艾美耳球虫(Eimeria tenella)对喹啉类抗球虫药产生抗药性的关系。[方法]收集安徽和县、舒城县、肥东县、庐江县、凤台县和全椒县6个地区的柔嫩艾美耳球虫,经分离、纯化、鉴定和鸡体试验法检测耐药性后,采用PCR法和DNA测序相结合的方法,分析各个地区的野外分离株及敏感株之间Cytb基因的序列突变情况。[结果]获得了1 100 bp的目的片段。测序后发现:与敏感株相比,耐药株中Cytb基因有更多的基因突变,主要表现为T→C、T→A、A→G的突变,其中以T→A的突变最为明显。其中一些碱基的突变引起了氨基酸的改变。[结论]这些碱基的突变有可能是导致柔嫩艾美耳球虫产生对癸氧喹酯抗药性的原因之一。
[Abstract]:[objective] to study the relationship between cytochrome b gene Cytb mutation and the resistance of Eimeria tenella) to quinoline anticoccidists. [methods] collected from Hehe County, Shucheng County, Feidong County, Lujiang County, Anhui Province. After isolation, purification, identification and chicken body test of Eimeria tenella in six areas of Fengtai County and Quanjiao County, the drug resistance was detected by PCR and DNA sequencing. The sequence mutation of Cytb gene between field isolates and sensitive strains in various regions was analyzed. [results] A target fragment of 1 100 BP was obtained. After sequencing, it was found that there were more mutations of Cytb gene in resistant strains than in susceptible strains. The main manifestation is T. 鈫扖,T. 鈫扐,A. 鈫扜 mutation, in which T. 鈫扵he mutation of A was the most obvious. Some of the base mutations caused amino acid changes. [conclusion] the mutation of these bases may be one of the reasons leading to the resistance of Eimeria tenella to decanoquinolide.
【作者单位】：安徽农业大学动物科技学院;
【基金】：安徽省教育厅项目(KJ2012A115)
【分类号】：S852.7

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