2015年云南省部分猪场主要病毒性疾病的病原学检测

发布时间：2018-03-20 18:15

本文选题：云南省　切入点：猪场　出处：《黑龙江畜牧兽医》2017年04期 　论文类型：期刊论文

【摘要】：为了解云南省猪群主要病毒性疾病感染情况,应用PCR与RT-PCR方法对2015年间云南省部分规模化猪场和养猪散户送检的1 719份临床病例样品进行主要病原的检测,包括猪繁殖与呼吸综合征病毒(PRRSV)、猪伪狂犬病病毒(PRV)、猪瘟病毒(CSFV)、猪圆环病毒2型(PCV-2)、猪细小病毒(PPV)、猪2型脑炎病毒(JEV)。结果表明:检测PCV-2的176份样品中,阳性数为38份,阳性率为21.59%;检测PRRSV的734份样品中,阳性数为140份,阳性率为19.07%;检测CSFV的726份样品中,阳性数为89份,阳性率为12.26%;检测PRV的71份样品中,阳性数为2份,阳性率为2.82%;检测PPV的10份样品中,阳性数为1份,阳性率为10.00%,检测JEV的2份样品中未检出阳性病例。其中有22份样品为CSFV与PRRSV混合感染;有13份样品为PRRSV与PCV-2混合感染;有7份样品为CSFV与PCV-2混合感染;各有1份为CSFV、PRRSV与PCV-2,PRRSV与PPV,PRRSV与PRV,CSFV、PRRSV与PRV混合感染。通过调查可了解当前云南猪场病毒性疾病的流行情况,从而为疫病的防控提供参考。
[Abstract]:In order to understand the infection status of the main viral diseases in swine herd in Yunnan Province, the main pathogens of 1,719 clinical cases were detected by PCR and RT-PCR methods in some large-scale pig farms and individual pig farmers in Yunnan Province in 2015. These include PRRSVV, PRV, CSFV, PCV-2, PPV and JEV. The results showed that 38 of the 176 samples for PCV-2 were positive. The positive rate was 21.59. Of the 734 samples tested for PRRSV, 140 were positive, and the positive rate was 19.07. of the 726 samples tested for CSFV, 89 were positive, the positive rate was 12.26. of the 71 samples tested for PRV, 2 were positive. The positive rate was 2.82%, the positive rate was 10.00 in 10 samples of PPV, and no positive cases were detected in 2 samples of JEV. Among them, 22 samples were mixed with CSFV and PRRSV, 13 samples were mixed infection of PRRSV and PCV-2. There were 7 samples of mixed infection of CSFV and PCV-2, and 1 case of mixed infection of PRRSV and PCV-2 PRRSV and PRRSV and PRRSV of PRV and PRV. The epidemic situation of viral diseases in Yunnan pig farm could be understood by investigation, thus providing a reference for the prevention and control of epidemic disease.
【作者单位】：云南农业职业技术学院;云南农业大学;
【基金】：云南省教育厅科学研究基金项目(YNAVC201303)
【分类号】：S858.28

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