肉鸡腹脂双向选择系中RNA编辑位点的鉴定研究

发布时间：2018-03-21 21:15

本文选题：肉鸡　切入点：脂肪组织　出处：《畜牧兽医学报》2017年09期 　论文类型：期刊论文

【摘要】：旨在准确获得肉鸡腹脂双向选择系脂肪组织中的RNA编辑位点信息,进而为脂肪相关研究提供更多的信息来源。本研究以东北农业大学肉鸡高、低腹脂双向选择品系第十九世代肉鸡为试验材料,使用转录组测序(RNA-seq)和基因组测序数据识别肉鸡腹部脂肪组织RNA编辑位点。通过生物信息学手段,设定严格的数据过滤与筛选策略,构建RNA编辑位点候选集。结果表明,在候选集中,通过对RNA编辑位点的分类和注释,在低脂系肉鸡脂肪组织中发掘转换型RNA编辑位点28个,颠换型RNA编辑位点101个,插入缺失型RNA编辑位点71个;在高脂系肉鸡脂肪组织中,发掘转换型RNA编辑位点30个,颠换型RNA编辑位点84个,插入缺失型RNA编辑位点77个。绝大多数RNA编辑位点落在内含子、基因间区域及基因上游区域(内含子区41.91%,基因间区域33.08%,基因下游20.59%,基因上游2.94%和外显子区1.47%)。试验验证了4个候选RNA编辑位点,其中3个位点落在与脂肪形成有重要关系的ATP1B3、SLC6A6和FLNB基因区域。本研究鉴定和验证了存在于肉鸡脂肪组织中的RNA编辑位点,为进一步研究RNA编辑影响脂肪组织生长发育的分子机制奠定了基础。
[Abstract]:The aim of this study was to obtain accurate information of RNA editing site in adipose tissue of bidirectional selection line of abdominal fat in broilers, and to provide more information sources for adipose related studies. Low abdominal fat bidirectional selection of the 19th generation broilers as experimental materials, using RNA-seq) and genomic sequencing data to identify RNA editing sites in abdominal adipose tissue of broilers. Strict data filtering and screening strategies were set up to construct the candidate set of RNA editing sites. The results showed that, by classifying and annotating RNA editing sites, 28 transformative RNA editing sites were found in adipose tissue of low-fat broilers. Among the adipose tissues of high-fat broilers, 30 were found to be transversive RNA editing sites, 84 were transversive RNA editing sites, and 101 were transversive RNA editing sites and 71 insertion deletion RNA editing sites, respectively. The majority of RNA editing sites fell in introns. Intergenic region and gene upstream region (intron region 41.91, intergenic region 33.08, downstream gene 20.5959, upstream 2.94% and exon 1.4775). Four candidate RNA editing sites were tested. Three of the loci were located in the region of ATP-1B3OSLC6A6 and FLNB gene, which were related to adipogenesis. The RNA editing site in adipose tissue of broiler was identified and verified. It provides a basis for further study on the molecular mechanism of RNA editing affecting the growth and development of adipose tissue.
【作者单位】：东北农业大学动物科学技术学院农业部鸡遗传育种重点实验室黑龙江省教育厅动物遗传育种与繁殖重点实验室;
【基金】：国家自然科学基金(31472088) 鸡重要经济性状的功能基因组学研究资助(863项目)(2013AA102501)
【分类号】：S831

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