猪流行性腹泻病毒反向遗传操作技术及其应用

发布时间：2018-03-25 12:18

本文选题：猪流行性腹泻病毒　切入点：反向遗传学　出处：《生物工程学报》2017年02期

【摘要】：猪流行性腹泻病毒(PEDV)是引起猪(特别是新生仔猪)急性、高度传染性消化道疾病的主要病原之一;2010年底以来,猪流行性腹泻(PED)的再次暴发给我国乃至全球养猪业造成了巨大经济损失。最近,研究者已先后建立了基于靶向RNA重组技术、细菌人工染色体(BAC)载体系统和体外连接技术的PEDV反向遗传学操作技术,为PEDV编码的蛋白质的功能、致病机制以及新型疫苗的研发开辟了新的思路。本文对PEDV反向遗传操作技术的研究进展及其在PEDV胰酶依赖性、S蛋白和ORF3蛋白的功能以及新一代疫苗研制等方面的应用现状进行了综述与展望。
[Abstract]:Porcine epidemic diarrhea virus (PEDVV) is one of the main pathogens causing acute and highly infectious digestive tract diseases in pigs (especially newborn piglets) since the end of 2010. The repeated outbreak of porcine epidemic diarrhea (PED) has caused huge economic losses to the pig industry in China and the world. Recently, researchers have established a recombinant technology based on targeted RNA. The PEDV reverse genetic manipulation of bacterial artificial chromosome PEDV vector system and in vitro ligation technique is the function of protein encoded by PEDV. The mechanism of pathogenesis and the research and development of new vaccines have opened up a new way of thinking. In this paper, the research progress of PEDV reverse genetic manipulation and its function in PEDV trypsin dependent protein S and ORF3 protein, as well as the development of new generation vaccine, etc. The present situation of application is summarized and prospected.
【作者单位】：上海市农业科学院畜牧兽医研究所;上海市农业遗传育种重点实验室;
【基金】：上海市科技支撑项目(No.13391901502) 国家自然科学基金(Nos.31402219,315725199) 上海市科技关农重点攻关项目(沪农科攻字(2015)第6-1-9号)资助~~
【分类号】：S852.65

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