麦洼牦牛GH、GHR、GHSR基因的SNPs检测及其与体尺性状的关联分析

发布时间：2018-03-26 14:57

本文选题：麦洼牦牛　切入点：GH基因　出处：《畜牧兽医学报》2017年04期

【摘要】：旨在探讨生长激素(Growth hormone,GH)、生长激素受体(Growth hormone receptor,GHR)、生长激素促分泌素受体(Growth hormone secretagogue receptor,GHSR)基因在麦洼牦牛中的遗传多样性,揭示不同基因型与其生长性状的关联性,同时为候选基因在牦牛中的表达调控提供理论依据,寻找可用于遗传育种辅助选择的分子标记。本试验采用DNA池技术,结合PCR-RFLP和直接测序法研究麦洼牦牛GH、GHR、GHSR基因的遗传多态性,分析候选基因多态位点与体高、体斜长、胸围、管围和体重等生长性状的关联性。结果表明:1)麦洼牦牛GH、GHR、GHSR基因均存在多态性,其中GH基因存在A757G和T949C2个SNPs位点,GHR基因发现T2416C、T3490C和A7500G3个突变位点;GHSR基因存在T1387C和T3006C突变;2)适合性检验表明,GHR基因A7500G位点在粉嘴类群,及GHSR基因T3006C位点在纯黑类群中偏离Hardy-Weinberg平衡状态,其他位点均符合Hardy-Weinberg平衡;3)差异显著性检验表明,GHR基因T2416C、T3490C和A7500G位点与麦洼牦牛管围极显著相关(P0.01),GHSR基因T1387C位点与其体重显著相关(P0.05)。麦洼牦牛GH、GHR、GHSR基因均存在遗传多态性,推断GHR基因T2416C、T3490C和A7500G位点、GHSR基因T1387C位点可能是影响麦洼牦牛管围、体重性状的主基因或与主基因相连锁的基因座,可作为辅助选择的遗传标记。
[Abstract]:The aim of this study was to investigate the genetic diversity of growth hormone growth hormone secretagogue receptor GHSRgene in Maiwa Yak, and to reveal the relationship between different genotypes and their growth traits. At the same time, it provides a theoretical basis for the regulation of candidate gene expression in yak, and looks for molecular markers that can be used for genetic and breeding assisted selection. The genetic polymorphism of the GHR-GHSR gene of GHHHR-GHSR gene was studied by DNA pool technique, PCR-RFLP and direct sequencing in Mai WA Yak. The relationship between candidate gene polymorphism and body height, body length, chest circumference, tube circumference and body weight was analyzed. The results showed that the GHR-GHSR gene was polymorphic in GHHR-GHSR gene of Maiwa Yak. Among them, there were A757G and T949C2 SNPs loci in GH gene. T2416CnT3490C and A7500G3 GHSR gene had T1387C and T3006C mutations. And the GHSR gene T3006C locus deviated from the Hardy-Weinberg equilibrium in the pure black group. All the other loci were consistent with Hardy-Weinberg balance 3 (P < 0.05). The results showed that there was a significant correlation between GHR T2416CnT3490C and A7500G loci and the tube circumference of Mai WA Yak (P0.01GHSR gene was significantly correlated with its body weight (P0.05). The genetic polymorphism of GHGHR-GHSR gene was found in Maiwa Yak. It is inferred that GHR gene T2416CfT3490C and A7500G locus T1387C may be the main genes affecting the tube circumference and body weight of Mai WA yak, or the loci linked to the main genes, which can be used as genetic markers for auxiliary selection.
【作者单位】：西南民族大学青藏高原研究院;西南民族大学动物遗传育种学国家民委-教育部重点实验室;西南民族大学青藏高原生态保护与畜牧业高科技研究示范基地;
【基金】：四川省科技计划项目(2015JY0248) 中央高校基本科研项目(2016NZYQN30) 国家肉牛牦牛产业技术体系(2017)
【分类号】：S823.85

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