H7N3亚型禽流感病毒反向遗传操作系统的建立

发布时间：2018-03-26 17:11

本文选题：禽流感病毒　切入点：亚型HN　出处：《中国动物传染病学报》2016年01期

【摘要】：为建立H7N3亚型禽流感病毒(Avian influenza virus,AIV)A/Duck/Zhejiang/690/2009(H7N3)(ZJ690)、A/Duck/Zhejiang/766/2009(H7N3)(ZJ766)反向遗传操作系统,本研究采用8质粒系统共转染293T细胞,成功拯救出了R-ZJ690和R-ZJ766 2株病毒。对获救病毒株进行全基因序列的测定,证实获救病毒的序列与亲本毒的序列完全一致。将H7N3亚型AIV亲本毒ZJ690株、ZJ766株和拯救病毒R-ZJ690株、R-ZJ766株均以106EID50剂量经鼻腔感染6周龄BALB/c小鼠,病毒均引起小鼠的体重下降但不造成死亡,感染后d4,仅在小鼠的鼻和肺中可以分离到病毒。由此可见,R-ZJ690、R-ZJ766与其亲本毒ZJ690株、ZJ766株保持了一致的生物学特性。本研究成功建立了H7N3 AIV ZJ690、ZJ766株的反向遗传操作系统,为H7亚型AIV的致病机理和跨宿主传播机制研究等奠定了基础。
[Abstract]:In order to establish the reverse genetic operating system of Avian influenza virus (Avian influenza virus) Ave / Duck / Zhejiang / 2009 H7N3 / ZJ690 / 766 / 2009 H7N3 / ZJ766), this study cotransfected 293T cells with eight plasmids and successfully saved the R-ZJ690 and R-ZJ766 2 strains. It was confirmed that the sequence of rescued virus was identical with that of parent virus. BALB/c mice of 6 weeks old were infected with H7N3 subtype AIV parent virus strain ZJ690 strain ZJ690 strain and rescue virus strain R-ZJ690 strain R-ZJ766 strain via nasal cavity with 106EID50 dose. The virus causes weight loss but does not cause death in mice. On the 4th day after infection, the virus could only be isolated from the nose and lungs of mice. It can be seen that R-ZJ690R-ZJ766 maintains the same biological characteristics as its parent virus ZJ690 strain ZJ766. In this study, the reverse genetic operating system of H7N3 AIV ZJ690 ZJ766 strain was successfully established. These results laid a foundation for the study of pathogenetic mechanism and transhost transmission mechanism of H7 subtype AIV.
【作者单位】：内蒙古农业大学兽医学院;中国农业科学院上海兽医研究所;
【基金】：自然科学基金(面上)项目(31472206)
【分类号】：S852.65

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