版纳微型猪近交系细胞色素P450家族基因CYP1A1克隆及生物信息学分析

发布时间：2018-03-26 19:31

本文选题：细胞色素P家族　切入点：CYPA基因　出处：《云南农业大学学报(自然科学)》2017年03期

【摘要】：【目的】克隆版纳微型猪近交系(Banna mini-pig inbred line,BMI)细胞色素P450家族基因CYP1A1的编码区序列,并对其进行生物信息学分析。【方法】利用RT-PCR方法获得CYP1A1基因的全长CDS序列,运用生物信息学对其核酸及蛋白序列进行分析,构建系统进化树。【结果】获得的BMI CYP1A1 CDS长度为1551 bp,编码516个氨基酸,蛋白质分子量为58.39 ku,等电点为7.83,位于内质网的概率是94.1%。CYP1A1蛋白质含有1个细胞色素P450半胱氨酸血红素配体位点,1个N-豆蔻酰化位点,1个酰胺化作用位点,2个N-糖基化位点、6个蛋白酶C磷酸化位点和5个蛋白激酶Ⅱ磷酸化位点。11个物种构建的系统进化分析表明,BMI(猪)CYP1A1与牛和山羊的关系最近。【结论】以上结果将为进一步研究版纳微型猪近交系CYP1A1基因功能奠定基础。
[Abstract]:[objective] to clone the coding region of cytochrome P450 family CYP1A1 of Banna mini-pig inbred lineage, Banna micropig inbred line, and analyze it by bioinformatics. [methods] the full-length CDS sequence of CYP1A1 gene was obtained by RT-PCR method. The nucleic acid and protein sequences were analyzed by bioinformatics, and the phylogenetic tree was constructed. The length of BMI CYP1A1 CDS was 1551 BP, encoding 516 amino acids. The molecular weight of the protein is 58.39 kuand the isoelectric point is 7.83. The probability of the protein being located in the endoplasmic reticulum is that the 94.1%.CYP1A1 protein contains one cytochrome P450 cysteine heme ligand site, one N-nutmeg acylation site, one amidation site, two N-glycosyl sites. The phylogenetic analysis of 11 species constructed indicates that the relationship between BMI-CYP1A1 and cattle and goat is the most recent. [conclusion] the above results will be further studied. The function of CYP1A1 gene of Banna miniature pig inbred line was established.
【作者单位】：云南农业大学云南省版纳微型猪近交系重点实验室;云南农业大学动物科学技术学院;
【基金】：国家自然科学基金项目(31660637,31460580,31660650)
【分类号】：S828.2

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