广西地方鸡微卫星位点LEI0258多态性研究

发布时间：2018-03-26 22:23

本文选题：广西地方鸡　切入点：微卫星　出处：《河南农业科学》2017年11期

【摘要】：通过微卫星位点LEI0258基因分型和基因测序,分析6个广西地方鸡品种的遗传变异并构建中介网络图,研究广西地方鸡的遗传多样性与分子进化。结果表明,120份样品经基因分型,检测到38个等位基因,长度为181~507 bp,等位基因205出现的频率最高(0.125),其次是275(0.113)、310(0.100)和251(0.067)。广西地方鸡保持着较高的遗传多样性水平,观察杂合度、期望杂合度和多态信息含量分别为0.800、0.943和0.936。经测序鉴定出39个等位基因,其中9个为首次发现。11个等位基因与已知的21种血清型相对应。基于侧翼序列变异信息的中介网络图显示,39个等位基因分为3个进化枝,红原鸡的部分基因存在于这些进化枝中,提示红原鸡可能是广西地方鸡的主要祖先。
[Abstract]:The genetic variation of 6 local chicken breeds in Guangxi was analyzed by LEI0258 genotyping and gene sequencing. The genetic diversity and molecular evolution of Guangxi local chickens were studied. The results showed that 120 samples were genotyped. 38 alleles were detected, the length of which was 181 ~ 507bp. the frequency of allele 205 was the highest 0.125, followed by 2750.113C310100.100) and 251P0.0670.The local chickens in Guangxi maintained a high level of genetic diversity and observed heterozygosity. The expected heterozygosity and polymorphic information content were 0.943 and 0.936, respectively. 39 alleles were identified by sequencing. Nine of the alleles were found for the first time, and 11 alleles corresponded to 21 known serotypes. The intermediary network based on the variation information of flanking sequence showed that 39 alleles were divided into 3 evolutionary branches. Some genes of Hongyuan chicken were found in these evolutionary branches, suggesting that Red Plain chicken may be the main ancestor of Guangxi local chicken.
【作者单位】：嘉应学院生命科学学院;
【基金】：广东省自然科学基金项目(2014A030307018) 广东省公益研究与能力建设项目(2015A020208020,2016A030303068) 嘉应学院省市共建重点建设项目(嘉院[2017]27号)
【分类号】：S831.2

【相似文献】